Acute effects of cadmium on liver phase I and phase II enzymes and metallothionein accumulation on sea bream Sparus aurata

被引：46
作者：
Bouraoui, Z. [2 ]
Banni, M. [1 ,2 ]
Ghedira, J. [2 ]
Clerandeau, C. [3 ]
Guerbej, H. [4 ]
Narbonne, J. F. [3 ]
Boussetta, H. [1 ,2 ]
机构：
[1] Higher Inst Hort, Lab Biochem & Ecotoxicol, Chott Mariem 4042, Sousse, Tunisia
[2] Inst Super Agron, Lab Biochim & Toxicol Environm, Chott Mariem, Sousse, Tunisia
[3] Univ Bordeaux 1, Lab Physicotoxico Chim Syst Nat, F-33405 Talence, France
[4] INSTM, Lab Marine Biotechnol, Monastir 5000, Tunisia
来源：
FISH PHYSIOLOGY AND BIOCHEMISTRY | 2008年 / 34卷 / 03期
关键词：
acute exposure; cadmium; EROD activity; GST activity; metallothionein;
D O I：
10.1007/s10695-007-9177-y
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This research was designed to study Sparus aurata (sea bream) biotransformation and detoxification responses to acute exposure to cadmium (Cd). Sexually immature gilthead sea bream were treated by intraperitoneal injection of Cd chloride (200 mu g kg(-1)) for 6, 12, 24 and 48 h. Cd accumulation was quantified in sea bream liver by graphite furnace atomic absorption spectroscopy after the various exposure periods. The following biological responses were measured: (1) ethoxyresorufin-O-deethylase (EROD) activity as phase I biotransformation parameter, (2) liver glutathione-S-transferase (GST) activity as a phase II conjugation enzyme and metallothionein (MT) content as specific response to Cd contamination. Cd bioaccumulation in the liver resulted in an increasing uptake up to 10.3 mu g g(-1) wet weight after 48 h of exposure. EROD showed a significant activation only after 6 h exposure and a return to control levels after 12 h. GST revealed significant activation starting from 12 h exposure. MT accumulation in liver showed the same behavior as GST activation.
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收藏
页码：201 / 207
页数：7
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