Molecular typing and genetic characterization of Mycobacterium avium subsp hominissuis isolates from humans and swine in Japan

被引：10
作者：
Adachi, Takashi [1 ,2 ]
Ichikawa, Kazuya [3 ]
Inagaki, Takayuki [3 ]
Moriyama, Makoto [4 ]
Nakagawa, Taku [1 ]
Ogawa, Kenji [1 ]
Hasegawa, Yoshinori [2 ]
Yagi, Tetsuya [5 ]
机构：
[1] Higashinagoya Natl Hosp, Natl Hosp Org, Dept Pulm Med, Meito Ku, 5-101 Umemorizaka, Nagoya, Aichi 4688620, Japan
[2] Nagoya Univ, Grad Sch Med, Dept Resp Med, Showa Ku, Nagoya, Aichi 4668560, Japan
[3] Nagoya Univ, Grad Sch Med, Dept Neuropsychopharmacol & Hosp Pharm, Showa Ku, Nagoya, Aichi 4668560, Japan
[4] Toyohashi Med Ctr, Natl Hosp Org, Dept Pharm, Toyohashi, Aichi 4408510, Japan
[5] Nagoya Univ, Grad Sch Med, Dept Infect Dis, Showa Ku, Nagoya, Aichi 4668560, Japan
来源：
JOURNAL OF MEDICAL MICROBIOLOGY | 2016年 / 65卷
关键词：
INTERNAL TRANSCRIBED SPACER; PULMONARY-DISEASE; VARIABLE-NUMBER; COMPLEX; STRAINS; IDENTIFICATION; SEQUENCE; IS1245; RELATEDNESS; DIVERSITY;
D O I：
10.1099/jmm.0.000351
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Mycobacterium avium subsp. hominissuis (MAH) causes disease in both humans and swine; however, the genetic variations in MAH isolates are unclear. The aim of this study was to elucidate the genetic variations in MAH isolates from humans and swine in Japan. We analysed the 16S-23S rDNA internal transcribed spacer (ITS) sequence and variable number of tandem repeats (VNTRs) using the Mycobacterium avium tandem repeat loci, prevalence of ISMav6 and clarithromycin resistance for MAH isolates from patients with pulmonary MAC (pMAC) disease (n=69), and HIV-seropositive and blood culture-positive (HIV-MAC) patients (n=28) and swine (n=23). In the minimum spanning tree based on VNTR analysis, swine MAC isolates belonged to a cluster distinguishable from that of human pMAC isolates. Isolates from HIV-MAC were scattered throughout both clusters. The three major distinct sequevars, Mav-A, Mav-B and Mav-F, were determined according to 16S-23S rDNA ITS sequence analysis in addition to three new sequevars, Mav-Q, Mav-R and Mav-S. Mav-A and Mav-F comprised the majority of human pMAC strains; in contrast, Mav-B predominated in swine isolates. Distribution of ITS sequevars in the minimum spanning tree based on VNTR analysis showed similar clusters of isolates from different origins, i.e. human pMAC, HIV-MAC and swine. These results, together with ISMav6 possession and clarithromycin resistance, revealed the genetic diversity of MAH strains recovered from humans and swine. Molecular epidemiology and genetic characterization in the present study showed the distinctive genetic evolutionary lineage of MAH strains isolated from human pMAC diseases and swine.
引用
收藏
页码：1289 / 1295
页数：7
相关论文
共 50 条
  • [21] Molecular Characterization of Mycobacterium avium subsp hominissuis of Two Groups of Lymph Nodes, Being Intradermal Tuberculin or Interferon-Gamma Test Positive and Negative, Isolated from Swiss Cattle at Slaughter
    Scherrer, Simone
    Landolt, Patricia
    Carroli, Natasha
    Stephan, Roger
    FRONTIERS IN VETERINARY SCIENCE, 2018, 5
  • [22] Comparison of Variable-Number Tandem-Repeat Markers typing and IS1245 Restriction Fragment Length Polymorphism fingerprinting of Mycobacterium avium subsp hominissuis from human and porcine origins
    Tirkkonen, Taneli
    Pakarinen, Jaakko
    Rintala, Elina
    Ali-Vehmas, Terhi
    Marttila, Harri
    Peltoniemi, Olli A. T.
    Makinen, Johanna
    ACTA VETERINARIA SCANDINAVICA, 2010, 52
  • [23] Comparative analysis of the genomes of clinical isolates of Mycobacterium avium subsp hominissuis regarding virulence-related genes
    Jeffrey, Brendan
    Rose, Sasha J.
    Gilbert, Kerrigan
    Lewis, Matthew
    Bermudez, Luiz E.
    JOURNAL OF MEDICAL MICROBIOLOGY, 2017, 66 (07) : 1063 - 1075
  • [24] Variable-Number Tandem-Repeat Analysis of Respiratory and Household Water Biofilm Isolates of "Mycobacterium avium subsp hominissuis" with Establishment of a PCR Database
    Iakhiaeva, Elena
    Howard, Susan T.
    Elliott, Barbara A. Brown
    McNulty, Steven
    Newman, Kristopher L.
    Falkinham, Joseph O., III
    Williams, Myra
    Kwait, Rebecca
    Lande, Leah
    Vasireddy, Ravikiran
    Turenne, Christine
    Wallace, Richard J., Jr.
    JOURNAL OF CLINICAL MICROBIOLOGY, 2016, 54 (04) : 891 - 901
  • [25] Genotyping of Mycobacterium avium subsp avium isolates from domestic animals in Slovenia by IS901 RFLP
    Pate, M.
    Moravkova, M.
    Krt, B.
    Pavlik, I.
    Ocepek, M.
    VETERINARNI MEDICINA, 2009, 54 (06) : 270 - 279
  • [26] The isolation and molecular characterization of Mycobacterium avium subsp paratuberculosis in Shandong province, China
    Yue, Ruichao
    Liu, Chunfa
    Barrow, Paul
    Liu, Fei
    Cui, Yongyong
    Yang, Lifeng
    Zhao, Deming
    Zhou, Xiangmei
    GUT PATHOGENS, 2016, 8
  • [27] Genotyping and strain distribution of Mycobacterium avium subspecies hominissuis isolated from humans and pigs in Belgium, 2011 2013
    Vluggen, C.
    Soetaert, K.
    Duytschaever, L.
    Denoel, J.
    Fauville-Dufaux, M.
    Smeets, F.
    Bruffaerts, N.
    Huygen, K.
    Fretin, D.
    Rigouts, L.
    Saegerman, C.
    Mathys, V.
    EUROSURVEILLANCE, 2016, 21 (03): : 18 - 25
  • [28] Mycobacterium avium subsp. paratuberculosis (MAP) molecular diversity in cattle, sheep, and goats from Latin America and the Caribbean: a systematic review
    Correa-Valencia, Nathalia M.
    Moyano, Roberto Damian
    Hernandez-Agudelo, Miguel
    Fernandez-Silva, Jorge A.
    TROPICAL ANIMAL HEALTH AND PRODUCTION, 2021, 53 (05)
  • [29] Typing of Mycobacterium avium Subspecies paratuberculosis Isolates from Newfoundland Using Fragment Analysis
    Podder, Milka P.
    Banfield, Susan E.
    Keefe, Greg P.
    Whitney, Hugh G.
    Tahlan, Kapil
    PLOS ONE, 2015, 10 (04):
  • [30] Molecular analysis and MIRU-VNTR typing of Mycobacterium avium subsp paratuberculosis strains from various sources
    Ronai, Z.
    Csivincsik, A.
    Gyuranecz, M.
    Kreizinger, Z.
    Dan, A.
    Janosi, S.
    JOURNAL OF APPLIED MICROBIOLOGY, 2015, 118 (02) : 275 - 283
12345