Growth and siderophore production of Xylella fastidiosa under iron-limited conditions

被引：27

作者：

Silva-Stenico, ME ^{[1
]}

Pacheco, FTH

Rodrigues, JLM

Carrilho, E

Tsai, SM

机构：

[1] Univ Sao Paulo, Ctr Energia Nucl Agr, Lab Biol Celular & Mol, Piracicaba, SP, Brazil

[2] Univ Sao Paulo, Inst Quim Sao Carlos, Lab Cromatog, Sao Carlos, SP, Brazil

来源：

MICROBIOLOGICAL RESEARCH | 2005年 / 160卷 / 04期

基金：

巴西圣保罗研究基金会;

关键词：

Chromeazurol S; iron chelation; Methylobacterium extorquens; Xylella fastidiosa; siderophores;

D O I：

10.1016/j.micres.2005.03.007

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In this study, the production of siderophores by Xylella fastidiosa from the citrus bacteria isolate 31b9a5c (FAPESP - ONSA, Brazil) was investigated. The preliminary evidence supporting the existence of siderophore in X. fastidiosa was found during the evaluation of sequencing data generated in our tab using the BLAST-X tool, which indicated putative open reading frames (ORFs) associated with iron-binding proteins. In an iron-Limited medium siderophores were detected in the supernatant of X. fastidiosa cultures. The endophytic bacterium Methylobacterium extorquens was also evaluated. Capillary electrophoresis was used to separate putative siderophores produced by X. fastidiosa. The bacterial culture supernatants of X. fastidiosa were identified negative for hydroxamate and catechol. and positive for M. extorquens that secreted hydroxamate-type siderophores. (c) 2005 Elsevier GmbH. All rights reserved.

引用

页码：429 / 436

页数：8

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