Suppression of Rice Stripe Virus Replication in Laodelphax striatellus Using Vector Insect-Derived Double-Stranded RNAs

被引：6

作者：

Fang, Ying ^{[1
]}

Choi, Jae Young ^{[1
]}

Park, Dong Hwan ^{[1
]}

Park, Min Gu ^{[1
]}

Kim, Jun Young ^{[1
]}

Wang, Minghui ^{[1
]}

Kim, Hyun Ji ^{[1
]}

Kim, Woo Jin ^{[1
]}

Je, Yeon Ho ^{[1
,2
]}

机构：

[1] Seoul Natl Univ, Coll Agr & Life Sci, Dept Agr Biotechnol, Seoul 08826, South Korea

[2] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 08826, South Korea

来源：

PLANT PATHOLOGY JOURNAL | 2020年 / 36卷 / 03期

关键词：

double-stranded RNA; Laodelphax striatellus; rice stripe virus; RNA interference; CATHEPSIN B-LIKE; SMALL BROWN PLANTHOPPER; PLANT-VIRUS; TRANSMISSION; OVEREXPRESSION; INTERFERENCE; PROTEINASE; RESISTANCE; IMMUNITY;

D O I：

10.5423/PPJ.OA.03.2020.0052

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

RNA interference (RNAi) has attracted attention as a promising approach to control plant viruses in their insect vectors. In the present study, to suppress replication of the rice stripe virus (RSV) in its vector, Laodelphax striatellus, using RNAi, dsRNAs against L. striatellus genes that are strongly upregulated upon RSV infection were delivered through a rice leaf-mediated method. RNAi-based silencing of peroxiredoxin, cathepsin B, and cytochrome P450 resulted in significant down regulation of the NS3 gene of RSV, achieving a transcriptional reduction greater than 73.6% at a concentration of 100 ng/mu l and, possibly compromising viral replication. L. striatellus genes might play crucial roles in the transmission of RSV; transcriptional silencing of these genes could suppress viral replication in L. striatellus. These results suggest effective RNAi-based approaches for controlling RSV and provide insight into RSV-L. striatellus interactions.

引用

页码：280 / 288

页数：9

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