Cytotoxicity, genotoxicity, and metal release in patients with fixed orthodontic appliances: A longitudinal in-vivo study

被引:106
作者
Hafez, Hend Salah [2 ]
Selim, Essam Mohamed Nassef
Eid, Faten Hussein Kamel
Tawfik, Wael Attia [3 ]
Al-Ashkar, Emad A. [4 ]
Mostafa, Yehya Ahmed [1 ]
机构
[1] Cairo Univ, Dept Orthodont & Dentofacial Orthoped, Fac Oral & Dent Med, Int Clin Res Grp, Giza, Egypt
[2] Natl Res Ctr, Oral & Dent Res Div, Dept Orthodont, Giza, Egypt
[3] Natl Res Ctr, Oral & Dent Res Div, Orthodont & Pedodont Dept, Giza, Egypt
[4] Natl Res Ctr, Div Phys, Dept Spect, Giza, Egypt
关键词
ORAL-MUCOSA CELLS; ION RELEASE; DNA-DAMAGE; STAINLESS-STEEL; BUCCAL CELLS; COMET ASSAY; NICKEL; ALLOYS; SALIVA; NITI;
D O I
10.1016/j.ajodo.2010.05.025
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Introduction: Treatment with fixed orthodontic appliances in the corrosive environment of the oral cavity warrants in-vivo investigations of biocompatibility. Methods: Eighteen control and 28 treated subjects were evaluated longitudinally. Four combinations of brackets and archwires were tested. Buccal mucosa cell samples were collected before treatment, and 3 and 6 months after appliance placement. The cells were processed for cytotoxicity, genotoxicity, and nickel and chromium contents. Results: In the treatment group, buccal mucosa cell viability values were 8.1% at pretreatment, and 6.4% and 4.5% at 3 and 6 months, respectively. The composite score, a calculated DNA damage value, decreased from 125.6 to 98.8 at 6 months. Nickel cellular content increased from 0.52 to 0.68 and 0.78 ng per milliliter, and chromium increased from 0.31 to 0.41 and 0.78 ng per milliliter at 3 and 6 months, respectively. Compared with the control group, the treated subjects showed significant differences for DNA damage and chromium content at 3 months only. Conclusions: Fixed orthodontic appliances decreased cellular viability, induced DNA damage, and increased the nickel and chromium contents of the buccal mucosa cells. Compared to the control group, these changes were not evident at 6 months, possibly indicating tolerance for or repair of the cells and the DNA. (Am J Orthod Dentofacial Orthop 2011;140:298-308)
引用
收藏
页码:298 / 308
页数:11
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