Construction of tissue microarrays from prostate needle biopsy specimens

被引：27

作者：

Jhavar, S

Corbishley, CM

Dearnaley, D

Fisher, C

Falconer, A

Parker, C

Eeles, R

Cooper, CS

机构：

[1] Inst Canc Res, Male Urol Canc Res Ctr, Sect Mol Carcinogenesis, Sutton SM2 5NG, Surrey, England

[2] Royal Marsden NHS Fdn Trust, Dept Acad Urol, London SW17 0QT, England

[3] Inst Canc Res, London SW17 0QT, England

[4] Royal Marsden NHS Fdn Trust, Dept Histopathol, London SW3 6JJ, England

[5] Inst Canc Res, Sect Canc Genet, Sutton SM2 5NG, Surrey, England

来源：

BRITISH JOURNAL OF CANCER | 2005年 / 93卷 / 04期

关键词：

prostate; cancer; needle biopsy; tissue microarray; immunohistochemistry;

D O I：

10.1038/sj.bjc.6602726

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Needle biopsies are taken as standard diagnostic specimens for many cancers, but no technique exists for the high-throughput analysis of multiple individual immunohistochemical (IHC) markers using these samples. Here we present a simple and highly reliable technique for constructing tissue microarrays (TMAs) from prostatic needle biopsies. Serial sectioning of the TMAs, called 'Checkerboard TMAs', facilitated expression analysis of multiple proteins using IHC markers. In total, 100% of the analysed biopsies within the TMA both preserved their antigenicity and maintained their morphology. Checkerboard TMAs will allow the use of needle biopsies (i) alongside other tissue specimens (trans-urethral resection of prostates and prostatectomies in the case of prostate cancer) in clinical correlation studies when searching for new prognostic markers, and (ii) in a diagnostic context for assessing expression of multiple proteins in cancers from patients prior to treatment.

引用

页码：478 / 482

页数：5

共 28 条

[1] Use of interphase fluorescence in situ hybridization in prostate needle biopsy specimens with isolated high-grade prostatic intraepithelial neoplasia as a predictor of prostate adenocarcinoma on follow-up biopsy [J].