In situ fabrication of aloe-like Au-ZnO micro/nanoarrays for ultrasensitive biosensing of catechol

Currently, the large-scale and controllable fabrication of nanostructures on substrates remains a great challenge for further practical applications. In this work, a novel 3D aloe-like Au-ZnO nanocomposite was designed for in situ synthesis on an ITO substrate, achieving real-time detection of trace catechol (CC) in water. A seed-assisted hydrothermal approach was proposed to control the crystal distribution and growth direction to build a ZnO aloe-like architecture. To eliminate the natural weak conductivity of ZnO, Au nanoparticles were further deposited on all ZnO arrays to construct Au-ZnO micro/nanostructures. The synergetic effects derived from the aloe-like ZnO with a large specific area and Au nanoparticles with high conductivity resulted in both high electrocatalysis and fast electron transfer in enzymatic reactions. After laccase immobilization, the as-prepared biosensor exhibited specific recognition of catechol among other dihydroxybenzenes and phenol with an ultrahigh sensitivity of 131 mu A mM(-1), as well as an extremely wide linear range from 75 nM to 1100 mu M and an ultralow detection limit of 25 nM. In addition, in the detection of real lake samples, this biosensor showed satisfactory anti-interference ability and provided reliable assay results.