Identification and characterization of EYD1, encoding an erythritol dehydrogenase in Yarrowia lipolytica and its application to bioconvert erythritol into erythrulose

被引：44

作者：

Carly, Frederic ^{[1
]}

Steels, SBastien ^{[2
]}

Telek, Samuel ^{[2
]}

Vandermies, Marie ^{[2
]}

Nicaud, Jean-Marc ^{[3
]}

Fickers, Patrick ^{[2
]}

机构：

[1] Univ Libre Bruxelles, Unite Biotechnol & Bioprocedes, Brussels, Belgium

[2] Univ Liege, Gembloux Agrobio Tech, Microbial Proc & Interact, TERRA Teaching & Res Ctr, Liege, Belgium

[3] Univ Paris Saclay, Micalis Inst, INRA, AgroParisTech, F-78350 Jouy En Josas, France

来源：

BIORESOURCE TECHNOLOGY | 2018年 / 247卷

关键词：

Yarrowia lipolytic; Erythritol; Erythrulose; Erythritol dehydrogenase; Erythritol-erythrulose bioconversion; SACCHAROMYCES-CEREVISIAE; YEAST; GLYCEROL; GENE; L-(S)-ERYTHRULOSE; TRANSKETOLASE; TRANSPORTERS; EXPRESSION; CHIRONS; MARKER;

D O I：

10.1016/j.biortech.2017.09.168

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

In this study, gene YALI0F01650g has been isolated and characterized. Several experimental evidences suggest that the identified gene, renamed EYD1, encodes an erythritol dehydrogenase. An efficient bioreactor process for the bioconversion of erythritol into erythrulose was also developed. Using constitutive expression of EYD1 in a Y. lipolytica mutant containing a disrupted EYK1 gene, which encodes erythrulose kinase, erythrulose could be synthesized from erythritol at a rate of 0.116 g/g(DCW). h and with a bioconversion yield of 0.64 g/g.

引用

页码：963 / 969

页数：7

共 39 条

[31] Comparative genomics of biotechnologically important yeasts [J].

Riley, Robert ;

Haridas, Sajeet ;

Wolfe, Kenneth H. ;

Lopes, Mariana R. ;

Hittinger, Chris Todd ;

Goeker, Markus ;

Salamov, Asaf A. ;

Wisecaver, Jennifer H. ;

Long, Tanya M. ;

Calvey, Christopher H. ;

Aerts, Andrea L. ;

Barry, Kerrie W. ;

Choi, Cindy ;

Clum, Alicia ;

Coughlan, Aisling Y. ;

Deshpande, Shweta ;

Douglass, Alexander P. ;

Hanson, Sara J. ;

Klenk, Hans-Peter ;

LaButti, Kurt M. ;

Lapidus, Alla ;

Lindquist, Erika A. ;

Lipzen, Anna M. ;

Meier-Kolthoff, Jan P. ;

Ohm, Robin A. ;

Otillar, Robert P. ;

Pangilinan, Jasmyn L. ;

Peng, Yi ;

Rokas, Antonis ;

Rosa, Carlos A. ;

Scheuner, Carmen ;

Sibirny, Andriy A. ;

Slot, Jason C. ;

Stielow, J. Benjamin ;

Sun, Hui ;

Kurtzman, Cletus P. ;

Blackwell, Meredith ;

Grigoriev, Igor V. ;

Jeffries, Thomas W. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (35) :9882-9887

[32]

Sambrook J., 1989, Molecular Cloning: A Laboratory, V2

[33] Deciphering how LIP2 and POX2 promoters can optimally regulate recombinant protein production in the yeast Yarrowia lipolytica [J].

Sassi, Hosni ;

Delvigne, Frank ;

Kar, Tambi ;

Nicaud, Jean-Marc ;

Crutz-Le Coq, Anne-Marie ;

Steels, Sebastien ;

Fickers, Patrick .

MICROBIAL CELL FACTORIES, 2016, 15

[34] L-(S)-ERYTHRULOSE - THE SYNTHESIS OF (R)-1,2,4-BUTANETRIOL AND OF SOME RELATED C-4 CHIRONS [J].

VANDEREYCKEN, E ;

DEWILDE, H ;

DEPREZ, L ;

VANDEWALLE, M .

TETRAHEDRON LETTERS, 1987, 28 (40) :4759-4760

[35] EYK1 encoding erythrulose kinase as a catabolic selectable marker for genome editing in the non-conventional yeast Yarrowia lipolytica [J].

Vandermies, Marie ;

Denies, Olivia ;

Nicaud, Jean-Marc ;

Fickers, Patrick .

JOURNAL OF MICROBIOLOGICAL METHODS, 2017, 139 :161-164

[36] STUDIES ON LACTAMS .81. ENANTIOSPECIFIC SYNTHESIS AND ABSOLUTE-CONFIGURATION OF SUBSTITUTED BETA-LACTAMS FROM D-GLYCERALDEHYDE ACETONIDE [J].

WAGLE, DR ;

GARAI, C ;

CHIANG, J ;

MONTELEONE, MG ;

KURYS, BE ;

STROHMEYER, TW ;

HEGDE, VR ;

MANHAS, MS ;

BOSE, AK .

JOURNAL OF ORGANIC CHEMISTRY, 1988, 53 (18) :4227-4236

[37] The production of l-erythrulose by the action of Acetobacter suboxydans upon erythritol [J].

Whistler, RL ;

Underkofler, LA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1938, 60 :2507-2508

[38] Comparing cellular performance of Yarrowia lipolytica during growth on glucose and glycerol in submerged cultivations [J].

Workman, Mhairi ;

Holt, Philippe ;

Thykaer, Jette .

AMB EXPRESS, 2013, 3 :1-9

[39] Food-related applications of Yarrowia lipolytica [J].

Zinjarde, Smita S. .

FOOD CHEMISTRY, 2014, 152 :1-10

← 1 2 3 4 →