Identification and characterization of EYD1, encoding an erythritol dehydrogenase in Yarrowia lipolytica and its application to bioconvert erythritol into erythrulose

被引：44

作者：

Carly, Frederic ^{[1
]}

Steels, SBastien ^{[2
]}

Telek, Samuel ^{[2
]}

Vandermies, Marie ^{[2
]}

Nicaud, Jean-Marc ^{[3
]}

Fickers, Patrick ^{[2
]}

机构：

[1] Univ Libre Bruxelles, Unite Biotechnol & Bioprocedes, Brussels, Belgium

[2] Univ Liege, Gembloux Agrobio Tech, Microbial Proc & Interact, TERRA Teaching & Res Ctr, Liege, Belgium

[3] Univ Paris Saclay, Micalis Inst, INRA, AgroParisTech, F-78350 Jouy En Josas, France

来源：

BIORESOURCE TECHNOLOGY | 2018年 / 247卷

关键词：

Yarrowia lipolytic; Erythritol; Erythrulose; Erythritol dehydrogenase; Erythritol-erythrulose bioconversion; SACCHAROMYCES-CEREVISIAE; YEAST; GLYCEROL; GENE; L-(S)-ERYTHRULOSE; TRANSKETOLASE; TRANSPORTERS; EXPRESSION; CHIRONS; MARKER;

D O I：

10.1016/j.biortech.2017.09.168

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

In this study, gene YALI0F01650g has been isolated and characterized. Several experimental evidences suggest that the identified gene, renamed EYD1, encodes an erythritol dehydrogenase. An efficient bioreactor process for the bioconversion of erythritol into erythrulose was also developed. Using constitutive expression of EYD1 in a Y. lipolytica mutant containing a disrupted EYK1 gene, which encodes erythrulose kinase, erythrulose could be synthesized from erythritol at a rate of 0.116 g/g(DCW). h and with a bioconversion yield of 0.64 g/g.

引用

页码：963 / 969

页数：7

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