Survey of Salmonella spp. in beef meat for export at slaughterhouses in Brazil

被引：0
|
作者：
Bier, Daniele [1 ]
Kich, Jalusa D. [3 ]
Duarte, Sabrina C. [3 ]
Silvan, Marcio R. [4 ]
Valsoni, Luiza M. [1 ]
Ramos, Carlos A. N. [5 ]
Rodrigues, Dalia P. [6 ]
Araujo, Flabio R. [2 ]
机构：
[1] Univ Catolica Dom Bosco, Curso Med Vet, Av Tamandare 6000, BR-79117900 Campo Grande, MS, Brazil
[2] Embrapa Gado Corte, Grp Prod Anim, Av Radio Maia 830, BR-79106550 Campo Grande, MS, Brazil
[3] Embrapa Suinos & Aves, Necleo Temat Sanidade Suinos, Rodovia BR-153 Km 110, BR-89715899 Concordia, SC, Brazil
[4] Embrapa Gado Leite, Setor Saude Anim & Qualidade Leite, Eugenio do Nascimento 610, BR-36038330 Juiz De Fora, MG, Brazil
[5] Univ Fed Mato Grosso do Sul, Fac Med Vet & Zootecnia FAMEZ, Av Senador Felinto Muller 2443, BR-79070900 Campo Grande, MS, Brazil
[6] Inst Oswaldo Cruz, Lab Enterobacterias, Av Brasil 4365, BR-21040900 Rio De Janeiro, RJ, Brazil
来源：
PESQUISA VETERINARIA BRASILEIRA | 2018年 / 38卷 / 11期
关键词：
Salmonella spp; beef meat; slaughterhouses; Brazil; serotype; antibiotic resistance; salmonellosis; skinning; cooling; carcass; cattle; ESCHERICHIA-COLI O157-H7; ANTIMICROBIAL RESISTANCE; MICROBIAL-CONTAMINATION; MICROBIOLOGICAL QUALITY; PCR; SUSCEPTIBILITY; CARCASSES; SPP; STANDARDIZATION; MICROORGANISMS;
D O I：
10.1590/1678-5150-PVB-5867
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The aim of the present study was to investigate the presence of Salmonella spp. in samples collected from beef meat at three points of the slaughter line (after skinning, washing and cooling) at three slaughterhouses in Brazil that export meat. Detection was based on ISO 6579:2002 and confirmed by PCR and qPCR. The isolates were typified using slide agglutination tests and PFGE. The antibiotic sensitivity profile was determined using the disk diffusion method. Contamination was detected in only one slaughterhouse. The overall frequency of contamination by Salmonella spp. was 6.7% of carcasses (6/90) and 2.6% of carcass surface samples (7/270). All isolates were confirmed by PCR and qPCR. The serological analysis and the PFGE showed a single profile: Typhimurium. The strains demonstrated 100% susceptibility to ampicillin, cefotaxime, ciprofloxacin, chloramphenicol, gentamicin and tetracycline. Positive carcasses after cooling pose a direct risk to consumers, since the meat is considered ready to be marketed after this process.
引用
收藏
页码：2037 / 2043
页数：7
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