Molecular cloning, characterization and expression profiles of three estrogen receptors in protogynous hermaphroditic orange-spotted grouper (Epinephelus coioides)

被引：33

作者：

Chen, Huapu ^{[1
,2
]}

Zhang, Yong ^{[1
,2
]}

Li, Shuisheng ^{[1
,2
]}

Lin, Miansheng ^{[1
,2
]}

Shi, Yu ^{[1
,2
]}

Sang, Qing ^{[1
,2
]}

Liu, Min ^{[3
]}

Zhang, Haifa ^{[4
]}

Lu, Danqi ^{[1
,2
]}

Meng, Zining ^{[1
,2
]}

Liu, Xiaochun ^{[1
,2
]}

Lin, Haoran ^{[1
,2
,5
]}

机构：

[1] Sun Yat Sen Univ, Sch Life Sci, Inst Aquat Econ Anim, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou 510275, Guangdong, Peoples R China

[3] Xiamen Univ, Coll Oceanog & Environm Sci, Key State Lab Marine Environm Sci, Xiamen 361005, Fujian, Peoples R China

[4] Guangdong Daya Bay Fisheries Dev Ctr, Huizhou 516081, Guangdong, Peoples R China

[5] Hainan Univ, Coll Ocean, Haikou 570228, Hainan, Peoples R China

来源：

GENERAL AND COMPARATIVE ENDOCRINOLOGY | 2011年 / 172卷 / 03期

基金：

美国国家科学基金会;

关键词：

Estrogen receptor; Epinephelus coioides; Sex reversal; Ovarian development; MESSENGER-RNA EXPRESSION; TROUT ONCORHYNCHUS-MYKISS; PROTANDROUS BLACK PORGY; EUROPEAN SEA BASS; RAINBOW-TROUT; ER-ALPHA; ACANTHOPAGRUS-SCHLEGELI; ONTOGENIC EXPRESSION; TISSUE DISTRIBUTION; GENE-EXPRESSION;

D O I：

10.1016/j.ygcen.2011.03.025

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Estrogen plays key roles in vertebrate reproductive system via estrogen receptors (ERs) as mediating pathways. In the present study, three full-length ERs cDNA sequences were isolated from a protogynous teleost, the orange-spotted grouper (Epinephelus coioides), and were 2235 bp for gER alpha, 1967 bp for gER beta 1 and 2158 bp for gER beta 2, respectively. Phylogenetic and amino acid alignment analyses showed that each gER was clustered in the corresponding taxonomic groups of the perciformes and exhibited high evolutional conservation in functional domains. RT-PCR revealed that gERs expressed at different levels in all the obtained tissues. gER alpha highly expressed in mature ovaries, gER beta 1 mainly expressed in immature ovaries and gER beta 2 varied greatly during ovarian development. During female to male sex reversal induced by 17 alpha-methyltestosterone (MT) implantation, gER alpha decreased gradually, gER beta 1 increased gradually, and gER beta 2 decreased firstly and recovered subsequently in male stage. The present study speculated the potential roles of gERs during female maturation and female to male sex reversal induced by MT in the protogynous grouper E. coioides. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：371 / 381

页数：11

共 53 条

[1] Zebrafish hox clusters and vertebrate genome evolution [J].