Ginsenoside Rg3 decelerates hERG K+ channel deactivation through Ser631 residue interaction

The human ether-a-go-go-related gene (hERG) cardiac K+ channels are one of the representative pharmacological targets for development of drugs against cardiovascular diseases such as arrhythmia. Panax ginseng has been known to have cardio-protective effects. However, little is known about the molecular mechanisms of how ginsenosides, the active ingredients in Panax ginseng, interact with hERG K+ channel proteins. In the present study, we first examined the effects of various ginsenosides on hERG K+ channel activity by expressing human a subunits in Xenopus oocytes. Among them ginsenoside Rg(3) (Rg(3)) most potently enhanced outward I-hERG and peak I-tail. Rg(3) induced a large persistent deactivating-tail current (Ideactivating-tail) and profoundly decelerated deactivating current decay in both concentration- and voltage-dependent manners. The EC50 for steady-state I-hERG, peak I-tail, and persistent Ideactivating-tail was 0.41 +/- 0.05, 0.61 +/- 0.11, and 0.36 +/- 0.04 mu M, respectively. Rg(3) actions were blocked by bepridil, a hERG K+ channel antagonist. Site-directed mutation of S631, which is located at the channel pore entryway, to S631C in hERG K+ channel abolished Rg(3) actions on hERG K+ channels. These results indicate that S631 residue of hERG K+ channel plays an important role in Rg(3)-mediated induction of a persistent Ideactivating-tail and in a deceleration of hERG K+ channel deactivation. (C) 2011 Elsevier B.V. All rights reserved.