Evaluation of a TaqMan real-time PCR for the detection of Theileria parva in buffalo and cattle

被引：13

作者：

Papli, Natasha ^{[1
]}

Landt, Olfert ^{[2
]}

Fleischer, Carola ^{[2
]}

Koekemoer, Jacobus O. ^{[1
]}

Mans, Ben J. ^{[1
]}

Pienaar, Ronel ^{[1
]}

Josemans, Antoinette ^{[1
]}

Zweygarth, Erich ^{[1
]}

Potgieter, Frederick ^{[1
]}

Latif, Abdalla A. ^{[1
]}

机构：

[1] Agr Res Council, Onderstepoort Vet Inst, ZA-0110 Onderstepoort, South Africa

[2] TIB MOLBIOL Syntheselabor GmbH, D-12103 Berlin, Germany

来源：

VETERINARY PARASITOLOGY | 2011年 / 175卷 / 3-4期

关键词：

Theileria parva; Corridor disease; Real-time PCR; TaqMan probe; Hybridization probe; 18s rRNA; Sensitivity; Specificity; SYNCERUS-CAFFER;

D O I：

10.1016/j.vetpar.2010.10.038

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A real-time PCR assay based on TaqMan probe chemistry was developed for the detection of Theileria parva DNA in blood samples. It uses a Theileria genus-specific PCR primer set and a T. parva-specific probe to amplify and hybridize with a species-specific part of the 18S rRNA gene of the parasite. The test was evaluated using positive and negative reference blood samples and shown to be specific for T. parva. Analytical sensitivity was determined by testing a dilution series of T. parva positive blood. It was shown to be able to detect parasitaemia as low as 2 x 10(-6)%. The Taqman assay results were also compared with that obtained with the real-time hybridization probe PCR assay, which is currently employed as the official test for the diagnosis of T. parva infections in buffalo and cattle and was shown to be equally sensitive. A panel of 1164 field samples was screened using both assays and 164 samples tested positive in both tests, indicating a good correlation. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：356 / 359

页数：4

共 9 条

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