Compensatory hyperinsulinemia in high-fat diet-induced obese mice is associated with enhanced insulin translation in islets

被引:21
作者
Kanno, Ayumi [1 ]
Asahara, Shun-ichiro [1 ]
Masuda, Katsuhisa [2 ]
Matsuda, Tomokazu [1 ]
Kimura-Koyanagi, Maki [1 ]
Seino, Susumu [3 ]
Ogawa, Wataru [1 ]
Kido, Yoshiaki [1 ,2 ]
机构
[1] Kobe Univ, Div Diabet & Endocrinol, Dept Internal Med, Grad Sch Med, Kobe, Hyogo 6500017, Japan
[2] Kobe Univ, Div Med Chem, Dept Biophys, Grad Sch Hlth Sci, Kobe, Hyogo 6540142, Japan
[3] Kobe Univ, Div Mol & Metab Med, Dept Physiol & Cell Biol, Grad Sch Med, Kobe, Hyogo 6500047, Japan
关键词
High-fat diet; Mouse islet; Insulin translation; BETA-CELL MASS; PROINSULIN BIOSYNTHESIS; GENE-EXPRESSION; GLUCOSE; PREVALENCE; MODULATION; SECRETION; SURVIVAL; ABLATION; RECEPTOR;
D O I
10.1016/j.bbrc.2015.02.024
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A high-fat diet (HF) is associated with obesity, insulin resistance, and hyperglycemia. Animal studies have shown compensatory mechanisms in pancreatic beta-cells after high fat load, such as increased pancreatic beta-cell mass, enhanced insulin secretion, and exocytosis. However, the effects of high fat intake on insulin synthesis are obscure. Here, we investigated whether insulin synthesis was altered in correlation with an HF diet, for the purpose of obtaining further understanding of the compensatory mechanisms in pancreatic beta-cells. Mice fed an HF diet are obese, insulin resistant, hyperinsulinemic, and glucose intolerant. In islets of mice fed an HF diet, more storage of insulin was identified. We analyzed insulin translation in mouse islets, as well as in INS-1 cells, using non-radioisotope chemicals. We found that insulin translational levels were significantly increased in islets of mice fed an HF diet to meet systemic demand, without altering its transcriptional levels. Our data showed that not only increased pancreatic beta-cell mass and insulin secretion but also elevated insulin translation is the major compensatory mechanism of pancreatic beta-cells. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码:681 / 686
页数:6
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