Inactivation of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824

被引：42
作者：
Green, EM [1 ]
Bennett, GN [1 ]
机构：
[1] RICE UNIV,DEPT BIOCHEM & CELL BIOL,HOUSTON,TX 77251
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1996年 / 57-8卷
关键词：
Clostridium acetobutylicum; aldehyde/alcohol dehydrogenase; solvent production; gene inactivation; homologous recombination;
D O I：
10.1007/BF02941702
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A nonreplicative plasmid containing an internal aad gene fragment has been integrated into the chromosome of Clostridium acetobutylicum ATCC 824. Transformation was accomplished by electroporation with relatively high concentrations of methylated plasmid DNA. Southern hybridization experiments revealed that integration occurred by single crossover homologous recombination inactivating the aad gene. Integrants were relatively stable after 25 generations. Inactivation of the aad gene drastically reduced solvent production. This result suggests that aldehyde/alcohol dehydrogenase (AAD) plays a important role in butanol production.
引用
收藏
页码：213 / 221
页数：9
相关论文
共 50 条
[21]   Quantitative proteomic analysis of the influence of lignin on biofuel production by Clostridium acetobutylicum ATCC 824 [J].
Raut, Mahendra P. ;
Couto, Narciso ;
Pham, Trong K. ;
Evans, Caroline ;
Noirel, Josselin ;
Wright, Phillip C. .
BIOTECHNOLOGY FOR BIOFUELS, 2016, 9
[22]   Metabolic Engineering of Clostridium acetobutylicum ATCC 824 for Isopropanol-Butanol-Ethanol Fermentation [J].
Lee, Joungmin ;
Jang, Yu-Sin ;
Choi, Sung Jun ;
Im, Jung Ae ;
Song, Hyohak ;
Cho, Jung Hee ;
Seung, Do Young ;
Papoutsakis, E. Terry ;
Bennett, George N. ;
Lee, Sang Yup .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2012, 78 (05) :1416-1423
[23]   Optimization of hydrogen and butanol production from agave guishe juice using Clostridium acetobutylicum ATCC824 [J].
Oliva-Rodriguez, A. ;
Morales-Martinez, T. K. ;
Rodriguez-De la Garza, J. A. ;
Medina-Morales, M. A. ;
Gonzalez, L. J. Rios .
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2025, 108 :206-214
[24]   Structural analysis of Clostridium acetobutylicum ATCC 824 glycoside hydrolase from CAZy family GH105 [J].
Germane, Katherine L. ;
Servinsky, Matthew D. ;
Gerlach, Elliot S. ;
Sund, Christian J. ;
Hurley, Margaret M. .
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2015, 71 :1100-1108
[25]   Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824 [J].
Kovacs, Katalin ;
Willson, Benjamin J. ;
Schwarz, Katrin ;
Heap, John T. ;
Jackson, Adam ;
Bolam, David N. ;
Winzer, Klaus ;
Minton, Nigel P. .
BIOTECHNOLOGY FOR BIOFUELS, 2013, 6
[26]   Genome-scale reconstruction and in silico analysis of the Clostridium acetobutylicum ATCC 824 metabolic network [J].
Lee, Joungmin ;
Yun, Hongseok ;
Feist, Adam M. ;
Palsson, Bernhard O. ;
Lee, Sang Yup .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2008, 80 (05) :849-862
[27]   Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824 [J].
Willson, Benjamin J. ;
Kovacs, Katalin ;
Wilding-Steele, Tom ;
Markus, Robert ;
Winzer, Klaus ;
Minton, Nigel P. .
BIOTECHNOLOGY FOR BIOFUELS, 2016, 9
[28]   Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824 [J].
Ehsaan, Muhammad ;
Kuit, Wouter ;
Zhang, Ying ;
Cartman, Stephen T. ;
Heap, John T. ;
Winzer, Klaus ;
Minton, Nigel P. .
BIOTECHNOLOGY FOR BIOFUELS, 2016, 9
[29]   Cloning, sequencing, and characterization of the gene encoding flagellin, flaC, and the post-translational modification of flagellin, FlaC, from Clostridium acetobutylicum ATCC824 [J].
Lyristis, M ;
Boynton, ZL ;
Petersen, D ;
Kan, Z ;
Bennett, GN ;
Rudolph, FB .
ANAEROBE, 2000, 6 (02) :69-79
[30]   Arabinose-Induced Catabolite Repression as a Mechanism for Pentose Hierarchy Control in Clostridium acetobutylicum ATCC 824 [J].
Servinsky, Matthew D. ;
Renberg, Rebecca L. ;
Perisin, Matthew A. ;
Gerlach, Elliot S. ;
Liu, Sanchao ;
Sund, Christian J. .
MSYSTEMS, 2018, 3 (05)
12345