Inactivation of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824

被引：41
|
作者：
Green, EM [1 ]
Bennett, GN [1 ]
机构：
[1] RICE UNIV,DEPT BIOCHEM & CELL BIOL,HOUSTON,TX 77251
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1996年 / 57-8卷
关键词：
Clostridium acetobutylicum; aldehyde/alcohol dehydrogenase; solvent production; gene inactivation; homologous recombination;
D O I：
10.1007/BF02941702
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A nonreplicative plasmid containing an internal aad gene fragment has been integrated into the chromosome of Clostridium acetobutylicum ATCC 824. Transformation was accomplished by electroporation with relatively high concentrations of methylated plasmid DNA. Southern hybridization experiments revealed that integration occurred by single crossover homologous recombination inactivating the aad gene. Integrants were relatively stable after 25 generations. Inactivation of the aad gene drastically reduced solvent production. This result suggests that aldehyde/alcohol dehydrogenase (AAD) plays a important role in butanol production.
引用
收藏
页码：213 / 221
页数：9
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