Cloning and expression of chicken granulocyte-macrophage colony stimulating factor (GMCSF) gene

被引：0
|
作者：
Chaturvedi, Uttara [1 ]
Kalim, Shahina [2 ]
Kumar, Rajiv [1 ]
Sawant, Pradeep [1 ]
Tiwari, Sangeeta [3 ]
Khurana, S. K. [4 ]
Sahoo, A. P. [1 ]
Palia, Sudesh [1 ]
Tiwari, Ashok Kumar [1 ]
机构：
[1] Indian Vet Res Inst, Mol Biol Lab, Dept Anim Biotechnol, Izatnagar 243122, Uttar Pradesh, India
[2] Bundelkhand Univ, Dept Biochem, Jhansi 284128, UP, India
[3] Rohilkhand Univ, MJP, Fac Anim Sci, Bareilly 243006, UP, India
[4] NRC Equines, Hisar 125001, Haryana, India
来源：
INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY | 2010年 / 48卷 / 12期
关键词：
Genetic adjuvant; GMCSF; PCR; T-CELL RESPONSES; DNA VACCINE; GM-CSF; DENDRITIC CELLS; IMMUNE-RESPONSE; CONSTRUCTS; PROTECTION; ADJUVANTS; VIRUS; IL-12;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Granulocyte-macrophage colony stimulating factor (GMCSF), a multifunctional cytokine can enhance immune responses when administered along with DNA vaccine. Aim of the present study was to clone and express the chicken GMCSF cytokine for use as 'genetic adjuvant'. Chicken GMCSF gene 435bp was amplified using specific primers in which restriction sites of BamHI and HindIII were at forward and reverse primers respectively. The PCR product was cloned into eukaryotic expression vector pcDNA 3.1(+) and clones were confirmed by restriction digestion and nucleotide sequencing. Functional activity of recombinant GMCSF was checked by expression of GMCSF specific mRNA in transfected Vero cells by RT-PCR of total RNA isolated from transfected Vero cells. The recombinant plasmid can be used as genetic adjuvant in chicken.
引用
收藏
页码：1175 / 1180
页数：6
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