Photocatalytic Inactivation Efficiency of Anatase Nano-TiO2 Sol on the H9N2 Avian Influenza Virus

被引：46
作者：
Cui, Haixin [1 ]
Jiang, Jianfang [1 ]
Gu, Wei [1 ]
Sun, Changjiao [1 ]
Wu, Donglai [2 ]
Yang, Tao [2 ]
Yang, Guochen [3 ]
机构：
[1] Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, Beijing, Peoples R China
[2] Chinese Acad Agr Sci, Harbin Vet Res Inst, Harbin, Peoples R China
[3] N Carolina Agr & Tech State Univ, Dept Nat Resources & Environm Design, Greensboro, NC 27411 USA
来源：
PHOTOCHEMISTRY AND PHOTOBIOLOGY | 2010年 / 86卷 / 05期
关键词：
PEROXOTITANIC ACID-SOLUTION; TIO2; THIN-FILMS; ESCHERICHIA-COLI; STERILIZATION; WATER; DETOXIFICATION; PARTICLES;
D O I：
10.1111/j.1751-1097.2010.00763.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This study was conducted to investigate efficiency of TiO(2)nanomaterial as a novel environment-friendly disinfectant to control avian influenza (AI) by its photochemical sterilization ability. Anatase nano-TiO(2)sol, a neutral, viscous aqueous colloid of 1.6% TiO2, was synthesized from peroxotitanic acid solution according to the Ichinose method. Transmission electron microscope images showed that the TiO(2)particles were spindle-shaped with an average size of 50 nm. X-ray diffraction patterns revealed that the crystal phase of TiO(2)particles was anatase type with photocatalytic effect. A photocatalytic film of nano-TiO(2)sol was tested as a means of inactivating H(9)N(2)avian influenza virus (AIV). Inactivation capabilities were examined with 365 nm ultraviolet (UV) radiation under black light by adjusting the UV intensity, the UV irradiation time and the quantity of AIV. The titer change of AIV was determined by hemagglutination tests. Cytopathic effect of Madin Darby canine kidney (MDCK) cells was monitored by inverted fluorescence microscope. The results showed that anatase nano-TiO(2)sol significantly inactivated AIV under UV irradiation of 365 nm. The inactivation of AIV viruses reached up to 100%. Therefore, anatase nano-TiO(2)sol is a potentially environment-friendly antivirus agent to prevent AI.
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页码：1135 / 1139
页数：5
相关论文
共 29 条
[1]   Photocatalytic bactericidal activity of TiO2 in aqueous suspensions of E-coli [J].
Bekbolet, M .
WATER SCIENCE AND TECHNOLOGY, 1997, 35 (11-12) :95-100
[2]   Photocatalytic detoxification with the thin-film fixed-bed reactor (TFFBR): Clean-up of highly polluted landfill effluents using a novel TiO2-photocatalyst [J].
Bekbolet, M ;
Lindner, M ;
Weichgrebe, D ;
Bahnemann, DW .
SOLAR ENERGY, 1996, 56 (05) :455-469
[3]   INCREMENT OF PHOTOCATALYTIC KILLING OF CANCER-CELLS USING TIO2 WITH THE AID OF SUPEROXIDE-DISMUTASE [J].
CAI, R ;
HASHIMOTO, K ;
KUBOTA, Y ;
FUJISHIMA, A .
CHEMISTRY LETTERS, 1992, (03) :427-430
[4]   Different inactivation Behaviors of MS-2 phage and Escherichia coli in TiO2 photocatalytic disinfection [J].
Cho, M ;
Chung, HM ;
Choi, WY ;
Yoon, JY .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (01) :270-275
[5]   Photocatalytic antimicrobial activity of thin surface films of TiO2, CuO and TiO2/CuO dual layers on Escherichia coli and bacteriophage T4 [J].
Ditta, Iram B. ;
Steele, Alex ;
Liptrot, Christopher ;
Tobin, Julie ;
Tyler, Helen ;
Yates, Heather M. ;
Sheel, David W. ;
Foster, Howard A. .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2008, 79 (01) :127-133
[6]   Optimization of hydroxyl radical formation using TiO2 as photocatalyst by response surface methodology [J].
Eremia, Sandra A. V. ;
Chevalier-Lucia, Dominique ;
Radu, Gabriel-Lucian ;
Marty, Jean-Louis .
TALANTA, 2008, 77 (02) :858-862
[7]  
Feng Yan-meng, 2004, Virologica Sinica, V19, P639
[8]   HETEROGENEOUS PHOTOCATALYTIC OXIDATION OF AROMATIC-COMPOUNDS ON TIO2 [J].
FUJIHIRA, M ;
SATOH, Y ;
OSA, T .
NATURE, 1981, 293 (5829) :206-208
[9]   Microbiological disinfection of water and air by photocatalysis [J].
Guillard, Chantal ;
Bui, Thu-Hoai ;
Felix, Caroline ;
Moules, Vincent ;
Lina, Bruno ;
Lejeune, Philippe .
COMPTES RENDUS CHIMIE, 2008, 11 (1-2) :107-113
[10]  
Han W, 2004, PROG BIOCHEM BIOPHYS, V31, P982
123