Structure of type II collagen from sturgeon cartilage and its effect on adjuvant-induced rheumatoid arthritis in rats

被引:2
作者
Li, Zhaoxia [1 ,2 ]
Bai, Xue [1 ]
Fan, Yan [1 ]
Jia, Qiannan [1 ]
Zhang, Hongwei [3 ]
Hou, Hu [1 ,2 ]
机构
[1] Ocean Univ China, Coll Food Sci & Engn, 5 YuShan Rd, Qingdao 266003, Shandong, Peoples R China
[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266071, Shandong, Peoples R China
[3] Technol Ctr Qingdao Customs, 83 Xinyue Rd, Qingdao 266109, Shandong, Peoples R China
基金
中国国家自然科学基金; 国家重点研发计划;
关键词
COMPLETE FREUNDS-ADJUVANT; NF-KAPPA-B; PROTEIN ANTIBODIES; MOUSE MODEL; FT-IR; SKIN; PURIFICATION; TOLERANCE; CYTOKINES; ACID;
D O I
10.1039/d1fo03929f
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The purpose of this paper was to extract and characterize type II collagen of sturgeon cartilage (SC-CII), and to explore the effects of taking SC-CII orally on rheumatoid arthritis (RA) in rats. SC-CII showed a triple-helix structure (RPN = 0.12), with d1 of 11.82 angstrom and d2 of 4.08 angstrom, which was analyzed by FT-IR, CD, XRD, and MS. It was constructed of the repeating tripeptide unit Gly-X-Y, where X and Y are generally Pro or Hyp, proved by amino acid composition and peptide mass fingerprinting. Furthermore, the effects of SC-CII on RA were evaluated. Ankle thickness was significantly decreased in SC-CII groups, with changes in lymphocyte proliferation also observed. Compared with the model control group, there was an evident decrease in TNF-alpha, IL-1 beta, COX-2, MCP-1, and TLR-4 mRNA levels, but no remarkable differences in APF, MMP-3, and MyD88 mRNA levels in the SC-CII groups. In addition, TNF-alpha, IL-1 beta, RF, Anti-CII Ab were significantly reduced in the SC-CII groups, proved by ELISA. Therefore, SC-CII showed alleviating effects on RA through the TLR4/MyD88-NF kappa B pathway.
引用
收藏
页码:6152 / 6165
页数:14
相关论文
共 66 条
[1]   Serologic Diagnosis of Rheumatoid Arthritis [J].
Allard-Chamard, Hugues ;
Boire, Gilles .
CLINICS IN LABORATORY MEDICINE, 2019, 39 (04) :525-+
[2]   Skipjack (Katsuwonus pelamis) elastin hydrolysate-derived peptides attenuate UVA irradiation-induced cell damage in human HaCaT keratinocytes [J].
Amakye, William Kwame ;
Yang, Liu ;
Yao, Maojin ;
Yuan, Erdong ;
Ren, Ruiwen ;
Ren, Jiaoyan .
FOOD FRONTIERS, 2021, 2 (02) :184-194
[3]   Scientific validation of anti-arthritic effect of Kashayams - A polyherbal formulation in collagen induced arthritic rats [J].
Aswathy, I. S. ;
Krishnan, Santhi ;
Peter, Jasmine ;
Sabu, Vidya ;
Helen, A. .
JOURNAL OF AYURVEDA AND INTEGRATIVE MEDICINE, 2021, 12 (01) :20-27
[4]  
Ausar SF, 2001, RHEUMATOL INT, V20, P138
[5]  
Bäcklund J, 2002, EUR J IMMUNOL, V32, P3776, DOI 10.1002/1521-4141(200212)32:12<3776::AID-IMMU3776>3.0.CO
[6]  
2-A
[7]   Purification and characterization of type II collagen from chick sternal cartilage [J].
Cao, Hui ;
Xu, Shi-Ying .
FOOD CHEMISTRY, 2008, 108 (02) :439-445
[8]   DMARDS and infections in rheumatoid arthritis [J].
Caporali, Roberto ;
Caprioli, Marta ;
Bobbio-Pallavicini, Francesca ;
Montecucco, Carlomaurizio .
AUTOIMMUNITY REVIEWS, 2008, 8 (02) :139-143
[9]   Normal structure, function, and histology of the spleen [J].
Cesta, Mark F. .
TOXICOLOGIC PATHOLOGY, 2006, 34 (05) :455-465
[10]   Oral Administration of Shark Type II Collagen Suppresses Complete Freund's Adjuvant-Induced Rheumatoid Arthritis in Rats [J].
Chen, Lijuan ;
Bao, Bin ;
Wang, Nanping ;
Xie, Jing ;
Wu, Wenhui .
PHARMACEUTICALS, 2012, 5 (04) :339-352