Array-based sensing of proteins using conjugated polymers

被引：239

作者：

Miranda, Oscar R.

You, Chang-Cheng

Phillips, Ronnie

Kim, Ik-Bum

Ghosh, Partha S.

Bunz, Uwe H. F.

Rotello, Vincent M.

机构：

[1] Univ Massachusetts, Dept Chem, Amherst, MA 01003 USA

[2] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2007年 / 129卷 / 32期

关键词：

D O I：

10.1021/ja0737927

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A sensor array comprising six functionalized poly(p-phenyleneethynylene) polymers was constructed and used to detect and identify protein analytes. The presence of proteins alters the fluorescence properties of the polymers, generating a distinct fluorescence response pattern for a given protein. Linear discriminant analyses accurately differentiate the patterns of 17 proteins at nano- to micromolar concentrations. An identification accuracy of 97% was obtained on the basis of the tests with 68 unknown protein samples from the training set.

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页码：9856 / +

页数：3

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