Modification of titanium alloy surfaces for percutaneous implants by covalently attaching laminin

被引：27

作者：

Gordon, David J. ^{[1
]}

Bhagawati, Dimpu D. ^{[1
]}

Pendegrass, Catherine J. ^{[1
]}

Middleton, Claire A. ^{[1
]}

Blunn, Gordon W. ^{[1
]}

机构：

[1] Royal Natl Orthopaed Hosp, Univ Coll London, Inst Orthopaed & Musculoskeletal Sci, Ctr Biomed Engn, Stanmore HA7 4LP, Middx, England

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2010年 / 94A卷 / 02期

关键词：

titanium alloy; keratinocytes; laminin; 332; silanization; focal adhesions; CO-CR-MO; EPITHELIAL-CELLS; FOCAL ADHESIONS; BASEMENT-MEMBRANE; SOLUBLE LAMININ-5; IN-VITRO; ATTACHMENT; HEMIDESMOSOMES; REHABILITATION; KERATINOCYTES;

D O I：

10.1002/jbm.a.32735

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Percutaneous implants require a seal at the skin interface. Laminin (L-332) is a component of the basement membrane, integral to epidermal attachment. To enhance the attachment of keratinocytes onto the surface of titanium alloy (Ti6Al4V), we attached L-332 onto the surface using silanization (L-332=Ti6Al4V). Iodinated L-332 was used to investigate protein attachment kinetics. L-332=Ti6Al4V remained attached after immersion in serum compared with adsorbed L-332. Cells from a keratinocyte line (HaCaT) grown on L-332=Ti6Al4V were significantly smaller (p < 0.05) with over a 20-fold increase in the number of adhesion plaques compared with nontreated Ti6Al4V control discs or with adsorbed L-332 surfaces. We have shown that it is possible to augment the surface of Ti6Al4V with L-332 and that this significantly increases the attachment of keratinocytes. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 94A: 586-593, 2010

引用

页码：586 / 593

页数：8

共 31 条

[1] Substratum roughness alters the growth, area, and focal adhesions of epithelial cells, and their proximity to titanium surfaces [J].