All-trans-retinoic acid nanodisks

被引：55

作者：

Redmond, Katherine A. ^{[1
]}

Nguyen, Thanh-Son ^{[1
]}

Ryan, Robert O. ^{[1
]}

机构：

[1] Childrens Hosp Oakland, Res Inst, Ctr Prevent Obes Cardiovasc Dis & Diabet, Oakland, CA 94609 USA

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2007年 / 339卷 / 1-2期

关键词：

all-trans-retinoic acid; apolipoprotein; nanodisk; phospholipid; cell culture; drug delivery;

D O I：

10.1016/j.ijpharm.2007.02.033

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Nanodisks are nanoscale, disk-shaped phospholipid bilayers whose edge is stabilized by association of apolipoprotein molecules. Self-assembled ND particles enriched with all-trans-retinoic acid (ATRA) (phospholipid:ATRA molar ratio = 5.5:1) were generated wherein all reaction components were solubilized. ATRA-ND migrated as a single band (Stokes' diameter similar to 20 nm) on native gradient polyacrylamide gel electrophoresis. ATRA, phospholipid and apolipoprotein co-eluted from a Sepharose 6B gel filtration column, consistent with stable integration of ATRA into the ND particle milieu. Spectroscopic analysis of ATRA-ND in buffer yielded an absorbance spectrum characteristic of ATRA. ATRA-ND mediated time-dependent inhibition of cultured HepG2 cell growth more effectively than free ATRA. The nanoscale size of the formulation particles and the stable integration of biologically active ATRA suggest ND represent a potentially useful vehicle for solubilization and in vivo delivery of ATRA. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：246 / 250

页数：5

共 27 条

[1] Apoptotic events induced by naturally occurring retinoids ATRA and 13-cis retinoic acid on human hepatoma cell lines Hep3B and HepG2 [J].