Enzyme-based multi-component optical nanoprobes for sequence-specific detection of DNA hybridization

被引：115

作者：

Li, Jiang ^{[1
,2
]}

Song, Shiping ^{[1
]}

Liu, Xinfeng ^{[1
]}

Wang, Lihua ^{[1
]}

Pan, Dun ^{[1
]}

Huang, Qing ^{[1
]}

Zhao, Yun ^{[2
]}

Fan, Chunhai ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China

[2] Sichuan Univ, Sch Life Sci, Chengdu 610064, Peoples R China

来源：

ADVANCED MATERIALS | 2008年 / 20卷 / 03期

关键词：

D O I：

10.1002/adma.200701918

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Seeing is believing. A novel multicomponent gold nanoparticle-based nanoprobe is assembled with a thiolated DNA detection probe, horseradish peroxidase (HRP), and bovine serum albumin (BSA). In this nanoprobe, the DNA detection probe is used to construct complexes for "sandwich" -based DNA detection, HRP translates this hybridization event into an enzymatic catalysis-based optical signal, while and BSA acts as a non-specific blocker.

引用

页码：497 / +

页数：5

共 33 条

[1] Enhanced stability of enzymes adsorbed onto nanoparticles [J].

Asuri, Prashanth ;

Karajanagi, Sandeep S. ;

Vertegel, Alexey A. ;

Dordick, Jonathan S. ;

Kane, Ravi S. .

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2007, 7 (4-5) :1675-1678

[2] Nanodiagnostics: A new frontier for clinical laboratory medicine [J].

Azzazy, Hassan M. E. ;

Mansour, Mai M. H. ;

Kazmierczak, Steven C. .

CLINICAL CHEMISTRY, 2006, 52 (07) :1238-1246

[3] Raman dye-labeled nanoparticle probes for proteins [J].

Cao, YC ;

Jin, RC ;

Nam, JM ;

Thaxton, CS ;

Mirkin, CA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (48) :14676-14677

[4] Oligonucleotide-modified screen-printed gold electrodes for enzyme-amplified sensing of nucleic acids [J].

Carpini, G ;

Lucarelli, F ;

Marrazza, G ;

Mascini, M .

BIOSENSORS & BIOELECTRONICS, 2004, 20 (02) :167-175

[5] COLLOIDAL GOLD AS A BIOCOMPATIBLE IMMOBILIZATION MATRIX SUITABLE FOR THE FABRICATION OF ENZYME ELECTRODES BY ELECTRODEPOSITION [J].

CRUMBLISS, AL ;

PERINE, SC ;

STONEHUERNER, J ;

TUBERGEN, KR ;

ZHAO, JG ;

HENKENS, RW .

BIOTECHNOLOGY AND BIOENGINEERING, 1992, 40 (04) :483-490

[6] Gold nanoparticles: Assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology [J].

Daniel, MC ;

Astruc, D .

CHEMICAL REVIEWS, 2004, 104 (01) :293-346

[7] Single-mismatch detection using gold-quenched fluorescent oligonucleotides [J].

Dubertret, B ;

Calame, M ;

Libchaber, AJ .

NATURE BIOTECHNOLOGY, 2001, 19 (04) :365-370

[8]

EASTON DF, 1995, AM J HUM GENET, V56, P265

[9] Nanoparticle-based detection in cerebral spinal fluid of a soluble pathogenic biomarker for Alzheimer's disease [J].

Georganopoulou, DG ;

Chang, L ;

Nam, JM ;

Thaxton, CS ;

Mufson, EJ ;

Klein, WL ;

Mirkin, CA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (07) :2273-2276

[10] PREPARATION AND CHARACTERIZATION OF AU COLLOID MONOLAYERS [J].

GRABAR, KC ;

FREEMAN, RG ;

HOMMER, MB ;

NATAN, MJ .

ANALYTICAL CHEMISTRY, 1995, 67 (04) :735-743

← 1 2 3 4 →