Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite.

被引：1

作者：

Kumagai, Takashi ^{[1
]}

Anada, Takahisa ^{[3
]}

Honda, Yoshitomo ^{[3
]}

Takami, Masamichi ^{[2
]}

Kamijo, Ryutaro ^{[2
]}

Shimauchi, Hidetoshi ^{[1
]}

Suzuki, Osamu ^{[3
]}

机构：

[1] Tohoku Univ, Grad Sch Dent, Div Periodontol & Endodontol, Sendai, Miyagi, Japan

[2] Showa Univ, Dept Biochem, Sch Dentistry, Tokyo, Japan

[3] Tohoku Univ, Grad Sch Dent, Div Craniofacial Funct Engn, Sendai, Miyagi, Japan

来源：

BIOCERAMICS, VOL 20, PTS 1 AND 2 | 2008年 / 361-363卷

关键词：

octacalcium phosphate; hydroxyapatite; osteoblast; BMP-2; differentiation;

D O I：

10.4028/www.scientific.net/KEM.361-363.1025

中图分类号：

TQ174 [陶瓷工业]; TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The present study was designed to investigate whether bone morphogenetic protein-2 (BMP-2) adsorbed onto octacalcium phosphate (OCP) and hydroxyapatite (HA) surfaces influences osteoblastic cell differentiation. Osteoblastic cell line, UAMS32, were Cultured on plastic plate that was coated of synthetic OCP and HA. BMP-2 was pre-adsorbed on these calcium phosphates at 4 degrees C or room temperature for 24h. The cells more proliferated on the BMP-2 pre-adsorbed OCP and HA than that of the controls (OCP and HA coating without BMP-2). ALP activities were higher on HA than on OCP when BMP-2 were pre-adsorbed at 4 degrees C than at room temperature. The results suggest that pre-adsorption of BMP-2 in different temperature affects osteoblastic cell differentiation, most probably through different adsorption state of BMP-2 on this calcium phosphate

引用

页码：1025 / +

页数：2

共 20 条

[11] BMP-2 functionalized PEDOT:PSS-based OECTs for stem cell osteogenic differentiation monitoring [J].

Decataldo, Francesco ;

Druet, Victor ;

Pappa, Anna-Maria ;

Tan, Ellasia ;

Savva, Achilleas ;

Pitsalidis, Charalampos ;

Inal, Sahika ;

Kim, Ji-Seon ;

Fraboni, Beatrice ;

Owens, Roisin M. ;

Iandolo, Donata .

FLEXIBLE AND PRINTED ELECTRONICS, 2019, 4 (04)

[12] Biodegradable polymer scaffolds loaded with low-dose BMP-2 stimulate periodontal ligament cell differentiation [J].

Skodje, Anders ;

Idris, Shaza Bushra M. ;

Sun, Yang ;

Bartaula, Sushma ;

Mustafa, Kamal ;

Finne-Wistrand, Anna ;

Wikesjoe, Ulf M. E. ;

Leknes, Knut N. .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2015, 103 (06) :1991-1998

[13] Osteogenic differentiation of rabbit mesenchymal stem cells in thermo-reversible hydrogel constructs containing hydroxyapatite and bone morphogenic protein-2 (BMP-2) [J].

Na, Kun ;

Kim, Sung won ;

Sun, Bo Kyung ;

Woo, Dae Gyun ;

Yang, Han Na ;

Chung, Hyung Min ;

Park, Keun Hong .

BIOMATERIALS, 2007, 28 (16) :2631-2637

[14] Enhancement of osteoblastic differentiation of mesenchymal stromal cells cultured by selective combination of bone morphogenetic protein-2 (BMP-2) and fibroblast growth factor-2 (FGF-2) [J].

Maegawa, Naoki ;

Kawamura, Kenji ;

Hirose, Motohiro ;

Yajima, Hiroshi ;

Takakura, Yoshinori ;

Ohgushi, Hajime .

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2007, 1 (04) :306-313

[15] Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line [J].

Liang, Wenna ;

Lin, Munan ;

Li, Xihai ;

Li, Candong ;

Gao, Bizheng ;

Gan, Huijuan ;

Yang, Zhaoyang ;

Lin, Xuejuan ;

Liao, Linghong ;

Yang, Min .

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2012, 30 (04) :889-895

[16] Micro-porous polyetheretherketone implants decorated with BMP-2 via phosphorylated gelatin coating for enhancing cell adhesion and osteogenic differentiation [J].

Wu, Jiuping ;

Li, Linlong ;

Fu, Chuan ;

Yang, Fan ;

Jiao, Zixue ;

Shi, Xincui ;

Ito, Yoshihiro ;

Wang, Zongliang ;

Liu, Qinyi ;

Zhang, Peibiao .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2018, 169 :233-241

[17] Enhancing Cell Proliferation and Osteogenic Differentiation of MC3T3-E1 Pre-osteoblasts by BMP-2 Delivery in Graphene Oxide-Incorporated PLGA/HA Biodegradable Microcarriers [J].

Fu, Chuan ;

Yang, Xiaoyu ;

Tan, Shulian ;

Song, Liangsong .

SCIENTIFIC REPORTS, 2017, 7

[18] Porous membrane with reverse gradients of PDGF-BB and BMP-2 for tendon-to-bone repair: In vitro evaluation on adipose-derived stem cell differentiation [J].

Min, Hyun Ki ;

Oh, Se Heang ;

Lee, Jong Min ;

Im, Gun Il ;

Lee, Jin Ho .

ACTA BIOMATERIALIA, 2014, 10 (03) :1272-1279

[19] Bone morphogenetic protein-2 and-4 (BMP-2 and-4) mediates fraxetin-induced maturation and differentiation in human osteoblast-like cell lines [J].

Kuo, PL ;

Huang, YT ;

Chang, CH ;

Chang, JK .

BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2006, 29 (01) :119-124

[20] Effect of Bone Morphogenetic Protein-2 (BMP-2) or Troglitazone, as an Inducer of Osteogenic Cells or Adipocytes, on Differentiation of a Bone Marrow Mesenchymal Progenitor Cell Line Established from Temperature-Sensitive (ts) Simian Virus (SV) 40 T-Antigen Gene Transgenic Mice [J].

Nishii, Naomi ;

Arai, Michitsugu ;

Yanai, Nobuaki ;

Togari, Akifumi ;

Nakabayashi, Toshikatsu .

BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2009, 32 (01) :10-17

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