Osteoblastic cell differentiation on BMP-2 pre-adsorbed octacalcium phosphate and hydroxyapatite.

被引：1
|
作者：
Kumagai, Takashi [1 ]
Anada, Takahisa [3 ]
Honda, Yoshitomo [3 ]
Takami, Masamichi [2 ]
Kamijo, Ryutaro [2 ]
Shimauchi, Hidetoshi [1 ]
Suzuki, Osamu [3 ]
机构：
[1] Tohoku Univ, Grad Sch Dent, Div Periodontol & Endodontol, Sendai, Miyagi, Japan
[2] Showa Univ, Dept Biochem, Sch Dentistry, Tokyo, Japan
[3] Tohoku Univ, Grad Sch Dent, Div Craniofacial Funct Engn, Sendai, Miyagi, Japan
来源：
BIOCERAMICS, VOL 20, PTS 1 AND 2 | 2008年 / 361-363卷
关键词：
octacalcium phosphate; hydroxyapatite; osteoblast; BMP-2; differentiation;
D O I：
10.4028/www.scientific.net/KEM.361-363.1025
中图分类号：
TQ174 [陶瓷工业]; TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
The present study was designed to investigate whether bone morphogenetic protein-2 (BMP-2) adsorbed onto octacalcium phosphate (OCP) and hydroxyapatite (HA) surfaces influences osteoblastic cell differentiation. Osteoblastic cell line, UAMS32, were Cultured on plastic plate that was coated of synthetic OCP and HA. BMP-2 was pre-adsorbed on these calcium phosphates at 4 degrees C or room temperature for 24h. The cells more proliferated on the BMP-2 pre-adsorbed OCP and HA than that of the controls (OCP and HA coating without BMP-2). ALP activities were higher on HA than on OCP when BMP-2 were pre-adsorbed at 4 degrees C than at room temperature. The results suggest that pre-adsorption of BMP-2 in different temperature affects osteoblastic cell differentiation, most probably through different adsorption state of BMP-2 on this calcium phosphate
引用
收藏
页码：1025 / +
页数：2
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