Comparison of the Cepheid Xpert Xpress Flu/RSV Assay to in-house Flu/RSV triplex real-time RT-PCR for rapid molecular detection of Influenza A, Influenza B and Respiratory Syncytial Virus in respiratory specimens

被引：24
作者：
Ho, Yolanda I. I. [1 ]
Wong, Ann H. [1 ]
Lai, Raymond W. M. [1 ]
机构：
[1] Prince Wales Hosp, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China
来源：
JOURNAL OF MEDICAL MICROBIOLOGY | 2018年 / 67卷 / 11期
关键词：
influenza A; influenza B; RSV; Xpert Xpress Flu/RSV assay; FluAB/RSV triplex assay; rapid diagnostics; ADULTS;
D O I：
10.1099/jmm.0.000841
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
This study compared the performance of the commercially available Xpert Xpress Flu/RSV assay to an in-house FluAB/RSV triplex real-time RT-PCR assay for the detection of influenza A/B viruses and respiratory syncitial virus (RSV) from both nasopharyngeal aspirate (NPA) and nasopharyngeal flocked swab (NPS). A total of 20 external quality assurance (EQA) samples and 172 clinical respiratory samples were tested prospectively using both the Xpert Xpress Flu/RSV assay and the in-house FluAB/RSV triplex assay. For the EQA samples, concordance rate was 100% when tested with both assays. For clinical samples, there was 100% agreement between the two assays for detection of influenza A and influenza B, 96.7% agreement for detection of RSV and 99.7% agreement for negative results. With a shortened turnaround time and good diagnostic performance, application of the Xpert Xpress Flu/RSV assay can facilitate patient triage for prompt implementation of infection control measures and management of high-risk patients during influenza epidemics.
引用
收藏
页码：1576 / 1580
页数：5
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