Determination of antibody affinity by ELISA. Theory

被引：55

作者：

Bobrovnik, SA ^{[1
]}

机构：

[1] AV Palladin Biochem Inst, Dept Mol Immunol, UA-01601 Kiev, Ukraine

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2003年 / 57卷 / 03期

关键词：

antigen-antibody interaction; affinity; ELISA; mathematical modeling;

D O I：

10.1016/S0165-022X(03)00145-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

New approaches for the measurement of antibody affinity by ELISA are suggested and considered theoretically. It was shown that not only more precise and more convenient in comparison to that suggested earlier, but also more informative graphical representation of the experimental data in the appropriate coordinate could be used for evaluation of antibody affinity. The following cases were considered: (i) determination of antibody affinity for one kind of univalent antibodies, (ii) determination of antibody affinity for one kind of bivalent antibodies, (iii) determination of antibody affinity for two kinds of univalent antibodies, which are in a mixture, and (iv) determination of antibody affinity for two kinds of bivalent antibodies, which are in a mixture. Advantages and disadvantages of the different approaches are discussed. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：213 / 236

页数：24

共 10 条

[1]

Bobrovnik S A, 1999, Ukr Biokhim Zh (1999), V71, P112

[2]

BOBROVNIK SA, 1999, UKR BIOKHIM ZH, V71, P97

[3] MEASUREMENTS OF THE TRUE AFFINITY CONSTANT IN SOLUTION OF ANTIGEN-ANTIBODY COMPLEXES BY ENZYME-LINKED IMMUNOSORBENT-ASSAY [J].

FRIGUET, B ;

CHAFFOTTE, AF ;

DJAVADIOHANIANCE, L ;

GOLDBERG, ME .

JOURNAL OF IMMUNOLOGICAL METHODS, 1985, 77 (02) :305-319

[4] EVALUATION OF DISSOCIATION-CONSTANTS OF ANTIGEN-ANTIBODY COMPLEXES BY ELISA [J].

KIM, BB ;

DIKOVA, EB ;

SHELLER, U ;

DIKOV, MM ;

GAVRILOVA, EM ;

EGOROV, AM .

JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 131 (02) :213-222

[5] PROPERTIES OF GRAPHICAL REPRESENTATIONS OF MULTIPLE CLASSES OF BINDING SITES [J].

KLOTZ, IM ;

HUNSTON, DL .

BIOCHEMISTRY, 1971, 10 (16) :3065-&

[6]

KLOTZ IM, 1946, ARCH BIOCHEM, V9, P109

[7] MATHEMATICAL THEORY OF IMMUNORADIOMETRIC (LABELED ANTIBODY) ASSAYS [J].

RODBARD, D ;

WEISS, GH .

ANALYTICAL BIOCHEMISTRY, 1973, 52 (01) :10-44

[8] THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONS [J].

SCATCHARD, G .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1949, 51 (04) :660-672

[9] INFLUENCE OF SOLID-PHASE ANTIGEN IN COMPETITION ENZYME-LINKED IMMUNOSORBENT ASSAYS (ELISAS) ON CALCULATED ANTIGEN-ANTIBODY DISSOCIATION-CONSTANTS [J].

SELIGMAN, SJ .

JOURNAL OF IMMUNOLOGICAL METHODS, 1994, 168 (01) :101-110

[10] MODIFICATION OF AN ELISA-BASED PROCEDURE FOR AFFINITY DETERMINATION - CORRECTION NECESSARY FOR USE WITH BIVALENT ANTIBODY [J].

STEVENS, FJ .

MOLECULAR IMMUNOLOGY, 1987, 24 (10) :1055-1060

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