In pursuit of sensitivity: Lessons learned from viral nucleic acid detection and quantification on the Raindance ddPCR platform

被引：9

作者：

Long, Samuel ^{[1
]}

机构：

[1] Frederick Natl Lab Canc Res, AIDS & Canc Virus Program, Frederick, MD 21702 USA

来源：

METHODS | 2022年 / 201卷

基金：

美国国家卫生研究院;

关键词：

Digital PCR; Virus; HIV; Cure research; Infectious diseases; PCR sensitivity; DNA; RNA; DROPLET DIGITAL PCR; POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; RESPIRATORY-SYNCYTIAL-VIRUS; GENE-EXPRESSION; CIRCULATING TUMOR; QUANTITATIVE PCR; ENTEROVIRUS RNA; RAPID DETECTION; DNA-POLYMERASE;

D O I：

10.1016/j.ymeth.2021.04.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Sensitive PCR detection of viral nucleic acids plays a critical role in infectious disease research, diagnosis and monitoring. In the context of SARS-CoV-2 detection, recent reports indicate that digital PCR-based tests are significantly more sensitive than traditional qPCR tests. Numerous factors can influence digital PCR reaction sensitivity. In this review, using a model for human HIV infection and the Raindance ddPCR platform as an example, we describe technical aspects that contribute to sensitive viral signal detection in DNA and RNA from tissue samples, which often harbor viral reservoirs and serve as better predictors of disease outcome and indicators of treatment efficacy.

引用

页码：82 / 95

页数：14

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