Investigation of the therapeutic effect of 5-aminolevulinic acid based photodynamic therapy on hepatocellular carcinoma

被引:5
作者
Ozten, Ozge [1 ]
Eskiler, Gamze Guney [2 ]
Sonmez, Fatih [3 ]
Yildiz, Mustafa Zahid [4 ]
机构
[1] Sakarya Univ Appl Sci, Inst Grad Educ, Dept Biomed Engn, TR-54187 Sakarya, Turkey
[2] Sakarya Univ, Fac Med, Dept Med Biol, Korucuk Campus, TR-54290 Sakarya, Turkey
[3] Sakarya Univ Appl Sci, Pamukova Vocat High Sch, TR-54055 Sakarya, Turkey
[4] Sakarya Univ Appl Sci, Fac Technol, Dept Elect Elect Engn, TR-54187 Sakarya, Turkey
基金
英国科研创新办公室;
关键词
Hepatocellular carcinoma; Photodynamic therapy; 5-Aminolevulic acid (5-ALA); Apoptosis; CANCER; COMBINATION;
D O I
10.1007/s10103-021-03398-8
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Hepatocellular carcinoma (HCC) is a heterogeneous type of cancer and current treatment options limit successful therapy outcomes. Photodynamic therapy (PDT) has attracted attention as an alternative approach in the treatment of different types of cancer. However, there is no study in the literature regarding the effect of PDT on HCC, in vitro. Therefore, the aim of this study was to determine the cytotoxic and apoptotic effects of 5-aminolevulinic acid (5-ALA)/PDT on two different HCC cell lines in terms of hepatitis B virus (HBV) infection. The therapeutic effects of 5-ALA-based PDT on HCC cell lines (Huh-7 and SNU-449) were evaluated by PpIX-fluorescence accumulation, WST-1 analysis, Annexin V analysis, and acridine orange/ethidium bromide staining after irradiation with different light doses through diode laser. The results showed that 1 mM 5-ALA displayed higher PpIX fluorescence in the SNU-449 cell line than the Huh-7 cell line after 4 h of incubation. After irradiation with different light doses (3, 6, 9, and 12 J/cm(2)), 5-ALA significantly reduced the proliferation of HCC cells and induced apoptotic cell death (p < 0.01). Furthermore, SNU-449 cells were more responsive to 5-ALA-based PDT than Huh-7 cells due to possibly its molecular features as well as viral HBV status. Our preliminary data obtained from this study may contribute to the development of 5-ALA/PDT-based treatment strategies in the treatment of HCC. However, this study could be improved by the elucidation of the molecular mechanisms of cell death induced by 5-ALA/PDT in HCC cells, the use of different photosensitizer, light sources, and in vivo experiments.
引用
收藏
页码:1325 / 1332
页数:8
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