α10:: A determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells

被引:548
作者
Elgoyhen, AB
Vetter, DE
Katz, E
Rothlin, CV
Heinemann, SF
Boulter, J
机构
[1] Univ Calif Los Angeles, Inst Brain Res, Dept Psychiat & Biobehav Sci, Los Angeles, CA 90095 USA
[2] Hatos Res Ctr Neuropsychopharmacol, Los Angeles, CA 90095 USA
[3] Univ Buenos Aires, Consejo Nacl Invest Cient & Tecn, Inst Invest Ingn Genet & Biol Mol, RA-1428 Buenos Aires, DF, Argentina
[4] Salk Inst Biol Studies, Mol Neurobiol Lab, La Jolla, CA 92037 USA
[5] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Biol, RA-1428 Buenos Aires, DF, Argentina
关键词
D O I
10.1073/pnas.051622798
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We report the cloning and characterization of rat alpha 10, a previously unidentified member of the nicotinic acetylcholine receptor (nAChR) subunit gene family. The protein encoded by the alpha 10 nAChR subunit gene is most similar to the rat alpha9 nAChR, and both alpha9 and alpha 10 subunit genes are transcribed in adult rat mechanosensory hair cells. Injection of Xenopus laevis oocytes with alpha 10 cRNA alone or in pairwise combinations with either alpha2-alpha6 or beta2-beta4 subunit cRNAs yielded no detectable ACh-gated currents. However, coinjection of alpha9 and alpha 10 cRNAs resulted in the appearance of an unusual nAChR subtype, Compared with homomeric alpha9 channels, the alpha9 alpha 10 nAChR subtype displays faster and more extensive agonist-mediated desensitization, a distinct current-voltage relationship, and a biphasic response to changes in extracellular Ca2+ ions, The pharmacological profiles of homomeric alpha9 and heteromeric alpha9 alpha 10 nAChRs are essentially indistinguishable and closely resemble those reported for endogenous cholinergic eceptors found in vertebrate hair cells, Our data suggest that efferent modulation of hair cell function occurs, at least in part, through heteromeric nAChRs assembled from both alpha9 and alpha 10 subunits.
引用
收藏
页码:3501 / 3506
页数:6
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