Mitochondrial pyruvate carrier regulates the lignocellulosic decomposition rate through metabolism in Ganoderma lucidum

被引:8
作者
Xu, Wenzhao [1 ]
Fan, Junpei [1 ]
Wang, Yihong [1 ]
Wang, Yunxiao [1 ]
Zhu, Jing [1 ]
Ren, Ang [1 ]
Yu, Hanshou [1 ]
Shi, Liang [1 ]
Zhao, Mingwen [1 ]
机构
[1] Nanjing Agr Univ, Coll Life Sci, Dept Microbiol, Key Lab Microbiol Engn Agr Environm,Minist Agr, Nanjing 210095, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
mitochondrial pyruvate carrier; lignocellulose; cellulase; laccase; Ganoderma lucidum; metabolism; ENZYMATIC-HYDROLYSIS; NEUROSPORA-CRASSA; EXPRESSION; INHIBITION; INDUCTION; REPRESSOR; XLNR;
D O I
10.1093/femsle/fnab088
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The activity of mitochondrial pyruvate carrier (MPC) can be modulated to regulate intracellular metabolism under different culture conditions. In Ganoderma lucidum, the role of MPC in regulating carbon sources remains unknown. By knocking down MPC genes (MPC1 and MPC2), this research found that the loss of MPC increased the growth rate of G. lucidum by similar to 30% in a medium with wood chips as a carbon source. Then cellulase and laccase activities were tested. Endoglucanase and laccase activity increased by similar to 50% and similar to 35%, respectively, in MPC knockdown mutants compared with that in the wild type strain. Finally, the expression levels of genes related to glycolysis were assayed, and the transcription levels of these enzymes were found to be increased by similar to 250% compared with the wild type strain. In conclusion, the regulation of intracellular metabolism by MPC provides a new way to improve the use of nondominant carbon sources such as lignocellulose.
引用
收藏
页数:8
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