Long Non-Coding RNA of Myocardial Infarction Associated Transcript (LncRNA-MIAT) Promotes Diabetic Retinopathy by Upregulating Transforming Growth Factor-β1 (TGF-β1) Signaling

被引：47
作者：
Li, Qian [1 ,2 ]
Pang, Lei [3 ]
Yang, Wei [2 ]
Liu, Xin [1 ]
Su, Guanfang [1 ]
Dong, Yu [2 ]
机构：
[1] Jilin Univ, Hosp 2, Dept Ophthalmol, Changchun, Jilin, Peoples R China
[2] Jilin Univ, Hosp 1, Dept Ophthalmol, Changchun, Jilin, Peoples R China
[3] Jilin Univ, Hosp 1, Dept Anesthesiol, Changchun, Jilin, Peoples R China
来源：
MEDICAL SCIENCE MONITOR | 2018年 / 24卷
关键词：
Diabetic Retinopathy; RNA; Long Noncoding; Transforming Growth Factor beta1; BETA; PREVALENCE;
D O I：
10.12659/MSM.911787
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Background: Long non-coding RNA of myocardial infarction associated transcript (lncRNA-MIAT) has a reported role in microvascular dysfunction. This study aimed to investigate the role of lncRNA-MIAT and its effects on transforming growth factor-beta 1 (TGF-beta 1) signaling in patients with diabetic retinopathy and in ARPE-19 adult retinal pigment epithelial cells in vitro. Material/Methods: Study participants provided plasma samples and included patients with non-proliferative diabetic retinopathy (n=52), patients with diabetes without diabetic retinopathy (n=63), and healthy controls (n=56). Plasma levels of lncRNA-MIAT and TGF-beta 1 were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Pearson correlation analysis was performed on the plasma data, and the diagnostic relevance of plasma levels of lncRNA-MIAT for diabetic retinopathy was evaluated by receiver operating characteristic (ROC) curve analysis. Cells of the human retinal pigment epithelial cell line, ARPE-19, were cultured in high glucose with construction and transfection of a MIAT expression plasmid vector. Viability of ARPE-19 cells was detected by the MTT assay and Western blot measured the expression levels of TGF-beta 1. Results: Plasma levels of lncRNA-MIAT were significantly increased in patients with diabetic retinopathy compared with patients with diabetes without diabetic retinopathy and with healthy controls. ARPE-19 cells cultured in a high glucose environment showed reduced cell viability and upregulation of lncRNA-MIAT expression. Conclusions: Increased plasma levels of lncRNA-MIAT were significantly associated with the presence of diabetic retinopathy, and increased expression of lncRNA-MIAT reduced the viability of ARPE-19 cells in vitro by upregulating TGF-beta 1 signaling.
引用
收藏
页码：9497 / 9503
页数：7
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