Localization of fractalkine and CX3CR1 mRNAs in rat brain:: does fractalkine play a role in signaling from neuron to microglia?

被引：290

作者：

Nishiyori, A

Minami, M

Ohtani, Y

Takami, S

Yamamoto, J

Kawaguchi, N

Kume, T

Akaike, A

Satoh, M ^{[1
]}

机构：

[1] Kyoto Univ, Fac Pharmaceut Sci, Dept Mol Pharmacol, Kyoto 6068304, Japan

[2] Kyoto Univ, Fac Pharmaceut Sci, Dept Pharmacol, Kyoto 6068304, Japan

来源：

FEBS LETTERS | 1998年 / 429卷 / 02期

关键词：

fractalkine; CX3C chemokine receptor; messenger RNA; neuron-microglia interaction; rat brain;

D O I：

10.1016/S0014-5793(98)00583-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Localization of the mRNAs for fractalkine, a CX3C chemokine, and far its receptor CX(3)CR1 was investigated in the rat brain. In situ hybridization study revealed that fractalkine mRNA was dominantly expressed in neuronal cells particularly in the olfactory bulb, cerebral cortex, hippocampus, caudate putamen and nucleus accumbens. In vitro study using enriched neuronal or glial culture supported the dominant expression of fractalkine mRNA in neurons. On the other hand, CX(3)CR1 mRNA was dominantly expressed in glial cells throughout the whole brain. The in vitro study suggested the cells expressing CX(3)CR1 mRNA are microglia, not astrocytes or neurons. Fractalkine appears to function as a signal molecule from neuron to microglia, (C) 1998 Federation of European Biochemical Societies.

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页码：167 / 172

页数：6

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