Reliable Genotypic Tropism Tests for the Major HIV-1 Subtypes

被引：33
作者：
Cashin, Kieran [1 ,2 ]
Gray, Lachlan R. [1 ,3 ]
Harvey, Katherine L. [1 ,2 ]
Perez-Bercoff, Danielle [4 ]
Lee, Guinevere Q. [5 ]
Sterjovski, Jasminka [1 ,3 ]
Roche, Michael [1 ,3 ]
Demarest, James F. [6 ]
Drummond, Fraser [7 ]
Harrigan, P. Richard [5 ]
Churchill, Melissa J. [1 ,8 ,9 ]
Gorry, Paul R. [1 ,2 ,3 ,10 ]
机构：
[1] Burnet Inst, Ctr Biomed Res, Melbourne, Vic 3004, Australia
[2] Univ Melbourne, Dept Microbiol & Immunol, Parkville, Vic 3010, Australia
[3] Monash Univ, Dept Infect Dis, Melbourne, Vic 3800, Australia
[4] Ctr Rech Publ Sante, Lab Retrovirol, L-1526 Luxembourg, Luxembourg
[5] BC Ctr Excellence HIV AIDS, Vancouver, BC Y6Z 1Y6, Canada
[6] ViiV Healthcare, Res Triangle Pk, NC 27709 USA
[7] ViiV Healthcare Australia, Abbotsford, WA 3067, Australia
[8] Monash Univ, Dept Med, Melbourne, Vic 3800, Australia
[9] Monash Univ, Dept Microbiol, Clayton, Vic 3800, Australia
[10] RMIT Univ, Coll Sci Engn & Hlth, Sch Appl Sci, Melbourne, Vic 3001, Australia
来源：
SCIENTIFIC REPORTS | 2015年 / 5卷
基金：
美国国家卫生研究院; 英国医学研究理事会; 澳大利亚研究理事会; 澳大利亚国家健康与医学研究理事会;
关键词：
IMMUNODEFICIENCY-VIRUS TYPE-1; CORECEPTOR USAGE PREDICTION; TREATMENT-NAIVE PATIENTS; AMINO-ACID CHANGES; VIROLOGICAL RESPONSE; EVOLVING EPIDEMIC; PHENOTYPIC ASSAY; VIRAL TROPISM; CXCR4; TROPISM; V3; LOOP;
D O I：
10.1038/srep08543
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Over the past decade antiretroviral drugs have dramatically improved the prognosis for HIV-1 infected individuals, yet achieving better access to vulnerable populations remains a challenge. The principal obstacle to the CCR5-antagonist, maraviroc, from being more widely used in anti-HIV-1 therapy regimens is that the pre-treatment genotypic "tropism tests'' to determine virus susceptibility to maraviroc have been developed primarily for HIV-1 subtype B strains, which account for only 10% of infections worldwide. We therefore developed PhenoSeq, a suite of HIV-1 genotypic tropism assays that are highly sensitive and specific for establishing the tropism of HIV-1 subtypes A, B, C, D and circulating recombinant forms of subtypes AE and AG, which together account for 95% of HIV-1 infections worldwide. The PhenoSeq platform will inform the appropriate use of maraviroc and future CCR5 blocking drugs in regions of the world where non-B HIV-1 predominates, which are burdened the most by the HIV-1 pandemic.
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页数：8
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