A Peptide Derived from G0/G1 Switch Gene 2 Acts as Noncompetitive Inhibitor of Adipose Triglyceride Lipase

被引：34

作者：

Cerk, Ines K. ^{[1
]}

Salzburger, Barbara ^{[1
]}

Boeszoermenyi, Andras ^{[1
]}

Heier, Christoph ^{[1
]}

Pillip, Christoph ^{[1
]}

Romauch, Matthias ^{[1
]}

Schweiger, Martina ^{[1
]}

Cornaciu, Irina ^{[1
]}

Lass, Achim ^{[1
]}

Zimmermann, Robert ^{[1
]}

Zechner, Rudolf ^{[1
]}

Oberer, Monika ^{[1
]}

机构：

[1] Graz Univ, Inst Mol Biosci, A-8010 Graz, Austria

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2014年 / 289卷 / 47期

基金：

奥地利科学基金会;

关键词：

HORMONE-SENSITIVE LIPASE; ENERGY-METABOLISM; TERMINAL REGION; MESSENGER-RNA; LIPOLYSIS; EXPRESSION; G0S2; TISSUE; MICE; IDENTIFICATION;

D O I：

10.1074/jbc.M114.602599

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The protein G(0)/G(1) switch gene 2 (G0S2) is a small basic protein that functions as an endogenous inhibitor of adipose triglyceride lipase (ATGL), a key enzyme in intracellular lipolysis. In this study, we identified a short sequence covering residues Lys-20 to Ala-52 in G0S2 that is still fully capable of inhibiting mouse and human ATGL. We found that a synthetic peptide corresponding to this region inhibits ATGL in a noncompetitive manner in the nanomolar range. This peptide is highly selective for ATGL and does not inhibit other lipases, including hormone-sensitive lipase, monoacylglycerol lipase, lipoprotein lipase, and patatin domain-containing phospholipases 6 and 7. Because increased lipolysis is linked to the development of metabolic disorders, the inhibition of ATGL by G0S2-derived peptides may represent a novel therapeutic tool to modulate lipolysis.

引用

收藏

页码：32559 / 32570

页数：12

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