Light scattering of human red blood cells during metabolic remodeling of the membrane

被引：39
作者：
Park, YongKeun [1 ,2 ]
Best-Popescu, Catherine A. [3 ]
Dasari, Ramachandra R. [1 ]
Popescu, Gabriel [4 ]
机构：
[1] MIT, George R Harrison Spect Lab, Cambridge, MA 02139 USA
[2] MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA
[3] Univ Illinois, Coll Med, Urbana, IL 61801 USA
[4] Univ Illinois, Beckman Inst Adv Sci & Technol, Dept Elect & Comp Engn, Urbana, IL 61801 USA
来源：
JOURNAL OF BIOMEDICAL OPTICS | 2011年 / 16卷 / 01期
基金：
美国国家卫生研究院; 美国国家科学基金会;
关键词：
light scattering; red blood cells; erythrocyte; quantitative phase microscopy; MECHANICAL-PROPERTIES; DIFFRACTION PHASE; MICROSCOPY; SPECTROSCOPY; DYNAMICS; FLUCTUATIONS; ERYTHROCYTES;
D O I：
10.1117/1.3524509
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We present the light scattering properties of individual human red blood cells (RBCs). We show that both the RBC static and dynamic scattering signals are altered by adenosine 5'-triphosphate (ATP)-driven membrane metabolic remodeling. To measure the light scattering signal from individual RBCs, we use diffraction phase microscopy together with a Fourier transform light scattering technique. RBC cytosolic ATPs are both chemically and metabolically depleted, and the corresponding scattering signals are compared with the light scattering signal of normal RBCs having physiologic levels of ATP. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3524509]
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页数：7
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