Screening of entomopathogenic deuteromycetes for activities on targets involved in degenerative diseases of the central nervous system

被引:36
作者
Schmidt, K
Li, Z
Schubert, B
Huang, B
Stoyanova, S
Hamburger, M
机构
[1] Univ Jena, Inst Pharm, D-07743 Jena, Germany
[2] Anhui Agr Univ, Res Ctr Entomogenous Fungi, Hefei 230026, Peoples R China
关键词
Deuteromycetes; Paecilomyces; Beauveria; Sporothrix; NMDA antagonistic activity; MAO inhibition; radical scavenging activity; neurotrophic activity; PC-12; cells;
D O I
10.1016/j.jep.2003.08.009
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A selection of 32 fungal strains, belonging to 8 genera of entomopathogenic Deuteromycetes collected in various provinces of China, were screened for activities on targets involved in degenerative diseases of the central nervous system. The strains were grown under various fermentation conditions, and a total of 256 different extracts were obtained. The bioassays included functional screens for NMDA antagonistic activity in stably transfected fibroblasts, for neuritogenic activities in PC-12 cells, and tests for MAO inhibitory and radical scavenging properties. Several extracts with promising activities were identified. Some Paecilomyces extracts induced pronounced axonal-like outgrowths in PC-12 cells. In Paecilomyces militaris RCEF 0095, the neuritogenic activity could be linked to yellow pigments. Three Beauveria and Paecilomyces strains showed radical scavenging properties, which could be localized in the extract by a bioautographic assay on TLC. An extract obtained from the mycelium of Paecilomyces tenuipes RCEF 0275 showed moderate MAO inhibitory activity, whereas extracts of Sporothrix chondracris RCEF 0187 antagonized NMDA receptor mediated cell toxicity. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:251 / 260
页数:10
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