Structural and functional diversity in Listeria ecell wall teichoic acids

被引：46

作者：

Shen, Yang ^{[1
]}

Boulos, Samy ^{[2
]}

Sumrall, Eric ^{[1
]}

Gerber, Benjamin ^{[1
]}

Julian-Rodero, Alicia ^{[1
]}

Eugster, Marcel R. ^{[1
]}

Fieseler, Lars ^{[3
]}

Nystrom, Laura ^{[2
]}

Ebert, Marc-Olivier ^{[4
]}

Loessner, Martin J. ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, Inst Food Nutr & Hlth, Lab Food Microbiol, Schmelzbergstr 7, CH-8092 Zurich, Switzerland

[2] Swiss Fed Inst Technol, Inst Food Nutr & Hlth, Lab Food Biochem, Schmelzbergstr 9, CH-8092 Zurich, Switzerland

[3] ZHAW Sch Life Sci & Facil Management, Einsiedlerstr 31, CH-8820 Wadenswil, Switzerland

[4] Swiss Fed Inst Technol, Lab Organ Chem, Vladmimir Prelog Weg 3, CH-8093 Zurich, Switzerland

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2017年 / 292卷 / 43期

基金：

瑞士国家科学基金会;

关键词：

CELL-WALL; MASS-SPECTROMETRY; BACTERIOPHAGE ENDOLYSINS; NMR-SPECTROSCOPY; BINDING DOMAIN; MONOCYTOGENES; VIRULENCE; OLIGOSACCHARIDES; IDENTIFICATION; PEPTIDOGLYCAN;

D O I：

10.1074/jbc.M117.813964

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Wall teichoic acids (WTAs) are the most abundant glycopolymers found on the cell wall of many Gram-positive bacteria, whose diverse surface structures play key roles in multiple biological processes. Despite recent technological advances in glycan analysis, structural elucidation of WTAs remains challenging due to their complex nature. Here, we employed a combination of ultra-performance liquid chromatography-coupled electrospray ionization tandem-MS/MS andNMRto determine the structural complexity of WTAs from Listeria species. Weunveiled more than 10 different types ofWTApolymers that vary in their linkage and repeating units. Disparity in GlcNAc to ribitol connectivity, as well as variable O-acetylation and glycosylation of GlcNAc contribute to the structural diversity of WTAs. Notably, SPR analysis indicated that constitution of WTA determines the recognition by bacteriophage endolysins. Collectively, these findings provide detailed insight into Listeria cell wall-associated carbohydrates, and will guide further studies on the structure-function relationship of WTAs.

引用

页码：17832 / 17844

页数：13

共 44 条

[1] OatA, a Peptidoglycan O-Acetyltransferase Involved in Listeria monocytogenes Immune Escape, Is Critical for Virulence [J].