Dissecting protein structure and function using directed evolution

被引：42

作者：

Yuen, Courtney M. ^{[1
]}

Liu, David R. ^{[1
]}

机构：

[1] Harvard Univ, Howard Hughes Med Inst, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

来源：

NATURE METHODS | 2007年 / 4卷 / 12期

关键词：

D O I：

10.1038/nmeth1207-995

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To characterize the contributions of individual amino acids to the structure or function of a protein, researchers have adopted directed evolution approaches, which use iterated cycles of mutagenesis and selection or screening to search vast areas of sequence space for sets of mutations that provide insights into the protein of interest.

引用

页码：995 / 997

页数：3

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