High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging

被引：27

作者：

Amador, Cristina Isabel ^{[1
]}

Stannius, Rune Overlund ^{[2
]}

Roder, Henriette Lyng ^{[1
]}

Burmolle, Mette ^{[1
]}

机构：

[1] Univ Copenhagen, Dept Biol, Copenhagen, Denmark

[2] Tech Univ Denmark, DTU Bioengn, Bacterial Interact & Evolut Grp, Lyngby, Denmark

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2021年 / 190卷

关键词：

Biofilm; High throughput assay; Mixed species; Reproducibility; Imaging; Cytation5; Crystal Violet; Fluorescence labelling; STAPHYLOCOCCUS-EPIDERMIDIS; OPTIMIZATION; COCULTURES; ADHERENCE; MULTIPLE; STRAINS; CELLS; MODEL;

D O I：

10.1016/j.mimet.2021.106343

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data. This high-throughput method produces more robust data and provides information about morphology and spatial species organization within the biofilm.

引用

页数：5

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