Insect-resistant transgenic Pinus radiata

被引：38
作者：
Grace, LJ [1 ]
Charity, JA [1 ]
Gresham, B [1 ]
Kay, N [1 ]
Walter, C [1 ]
机构：
[1] New Zealand Forest Res Inst Ltd, Rotorua, New Zealand
来源：
PLANT CELL REPORTS | 2005年 / 24卷 / 02期
关键词：
biolistic transformation; embryogenic tissue; cry1Ac; conifers;
D O I：
10.1007/s00299-004-0912-x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Transgenic radiata pine (Pinus radiata D. Don) plants containing a Bacillus thuringiensis (Bt) toxin gene, crylAc, were produced by means of biolistic transformation of embryogenic tissue. Using the selectable marker gene nptII and corresponding geneticin selection, 20 independent transgenic lines from five genotypes were established. Over 200 plants regenerated from ten transgenic lines were successfully transferred to soil. The integration and expression of the introduced genes in transgenic tissue and/or plants were confirmed by PCR, Southern hybridisation and neomycin phosphotransferase II (NPTII) and Bt ELISA assays. Bioassays with larvae of the painted apple moth, Teia anartoides, demonstrated that transgenic plants displayed variable levels of resistance to insect damage, with one transgenic line being highly resistant to feeding damage.
引用
收藏
页码：103 / 111
页数：9
相关论文
共 46 条
  • [21] BETA-GLUCURONIDASE FROM ESCHERICHIA-COLI AS A GENE-FUSION MARKER
    JEFFERSON, RA
    BURGESS, SM
    HIRSH, D
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (22) : 8447 - 8451
  • [22] Larix laricina (tamarack): Somatic embryogenesis and genetic transformation
    Klimaszewska, K
    Devantier, Y
    Lachance, D
    Lelu, MA
    Charest, PJ
    [J]. CANADIAN JOURNAL OF FOREST RESEARCH, 1997, 27 (04) : 538 - 550
  • [23] Klimaszewska K, 2001, IN VITRO CELL DEV-PL, V37, P748
  • [24] PEMU - AN IMPROVED PROMOTER FOR GENE-EXPRESSION IN CEREAL CELLS
    LAST, DI
    BRETTELL, RIS
    CHAMBERLAIN, DA
    CHAUDHURY, AM
    LARKIN, PJ
    MARSH, EL
    PEACOCK, WJ
    DENNIS, ES
    [J]. THEORETICAL AND APPLIED GENETICS, 1991, 81 (05) : 581 - 588
  • [25] An improved procedure for production of white spruce (Picea glauca) transgenic plants using Agrobacterium tumefaciens
    Le, VQ
    Belles-Isles, J
    Dusabenyagasani, M
    Tremblay, FM
    [J]. JOURNAL OF EXPERIMENTAL BOTANY, 2001, 52 (364) : 2089 - 2095
  • [26] Agrobacterium tumefaciens-mediated transformation of hybrid larch (Larix kaempferi x L-decidua) and transgenic plant regeneration
    Levee, V
    Lelu, MA
    Jouanin, L
    Cornu, D
    Pilate, G
    [J]. PLANT CELL REPORTS, 1997, 16 (10) : 680 - 685
  • [27] Stable genetic transformation of white pine (Pinus strobus L.) after cocultivation of embryogenic tissues with Agrobacterium tumefaciens
    Levée, V
    Garin, E
    Klimaszewska, K
    Séguin, A
    [J]. MOLECULAR BREEDING, 1999, 5 (05) : 429 - 440
  • [28] Potential risk of accidental introduction of Asian gypsy moth (Lymantria dispar) to Australasia: effects of climatic conditions and suitability of native plants
    Matsuki, M.
    Kay, M.
    Serin, J.
    Floyd, R.
    Scott, John K.
    [J]. AGRICULTURAL AND FOREST ENTOMOLOGY, 2001, 3 (04) : 305 - 320
  • [29] Evaluation of an ELISA Assay for Rapid Detection and Quantification of Neomycin Phosphotransferase II in Transgenic Plants
    Nagel, Roland J.
    Manners, John M.
    Birch, Robert G.
    [J]. PLANT MOLECULAR BIOLOGY REPORTER, 1992, 10 (03) : 263 - 272
  • [30] Recent advances in the genetic transformation of trees
    Peña, L
    Séguin, A
    [J]. TRENDS IN BIOTECHNOLOGY, 2001, 19 (12) : 500 - 506
12345