Biodegradation of phenanthrene and pyrene by Ganoderma lucidum

Biodegradation of two polycyclic aromatic hydrocarbons (PAHs), phenanthrene and pyrene, by a white rot fungus, Ganoderma lucidum, in broth cultures was investigated. It was found that the biomass of the organism decreased with the increase of PAH concentration in the cultures. In the cultures with 2 to 50 mg l(-1) PAHs, the degradation rate constants (k(1)) increased with the PAH concentration, whereas, at the level of 100 mg l(-1), the degradation rate constants decreased. In the presence of 20 mg l(-1) PAHs, the highest degradation rates of both PAHs occurred in cultures with an initial pH of 4.0 at 30 degrees C. The addition of CuSO4, citric acid, gallic acid, tartaric acid, veratryl alcohol, guaiacol, 2,2'-azino-bis-(3-ethylbenzothazoline-6-sulfonate) (ABTS) enhanced the degradation of both PAHs and laccase activities; whereas the supplement of oxalate, di-n-butyl phthalate (DBP), and nonylphenol (NP) decreased the degradation of both PAHs and inhibited laccase production. In conclusion, G. lucidum is a promising white rot fungus to degrade PAHs such as phenanthrene and pyrene in the environment. (C) 2010 Elsevier Ltd. All rights reserved.