The effect of needle size inducing degeneration in the rat caudal disc: evaluation using radiograph, magnetic resonance imaging, histology, and immunohistochemistry

被引:93
作者
Keorochana, Gun [1 ,2 ]
Johnson, Jared S. [2 ]
Taghavi, Cyrus E. [2 ]
Liao, Jen-Chung [2 ,3 ]
Lee, Kwang-Bok [2 ,4 ]
Yoo, Jeong Hyun [2 ]
Ngo, Stephanie S. [2 ]
Wang, Jeffrey C. [2 ]
机构
[1] Mahidol Univ, Ramathibodi Hosp, Dept Orthopaed, Bangkok 10400, Thailand
[2] Univ Calif Los Angeles, Dept Orthopaed Surg, Los Angeles, CA 90404 USA
[3] Chang Gung Univ, Chang Gung Mem Hosp, Dept Orthopaed Surg, Tao Yuan 333, Taiwan
[4] Chonbuk Natl Univ Hosp, Chonbuk Natl Univ Med Sch, Dept Orthopaed Surg, Jeonju 561712, South Korea
关键词
Disc degeneration; Rat caudal disc; Different needle sizes; Cellular and matrix changes; Needle puncture; LUMBAR INTERVERTEBRAL DISC; NUCLEUS PULPOSUS; GENE-EXPRESSION; IN-VIVO; ANNULAR INJURY; PORCINE MODEL; PUNCTURE; CELLS; SPINE; TRANSPLANTATION;
D O I
10.1016/j.spinee.2010.08.013
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
BACKGROUND CONTEXT: The rat caudal disc has been increasingly used in studying of disc degeneration because of its simplicity, low cost, and efficiency. However, the reproducibility and standardization are essential to facilitate the investigations of biologic therapeutics at different stages of degeneration. PURPOSE: To identify the effect of different needle gauges to the degenerative response in rat caudal discs and to examine its pathogenesis by looking at the cellular and matrix changes. STUDY DESIGN: In vivo study of injury-induced rat caudal disc degeneration using needle puncture. PATIENT SAMPLE: Thirty-six Lewis rats aged 12-14 weeks. OUTCOME MEASURES: The induced degenerative discs were analyzed by plain radiograph, magnetic resonance imaging (MRI) and histological examination. Proteoglycan content was assessed by alcian blue stain. Immunohistochemistry using aggrecan, collagen II, and Sox-9 was also evaluated to investigate cell differentiation and matrix changes. METHODS: All rats were divided into three groups according to different needle gauges (180, 20G, and 220). Caudal discs were punctured percutaneously under image guidance. Radiographs and MRI were obtained at 2 weeks interval until 8 weeks. At each time point, three rats from each group were sacrificed for histological analysis and immunohistochemistry. RESULTS: Larger needle gauges, especially 180, produced more deterioration of the disc when compared with smaller sizes, particularly with time. Significant differences were identified in almost all parameters compared between 180 and 220 at the 8-week time point. For the effect of time in the same needle size, the differences occurred between 2- or 4-week and 8-week time point in the 18G and 20G groups. The proteoglycan and aggrecan stain gradually decreased over time. Chondrogenic differentiation was identified within the degenerative disc by detecting Sox-9 positive cells and collagen II accumulation increased as degeneration progressed. CONCLUSIONS: The puncture-induced degenerative changes in rat caudal discs can imitate the human degenerative cascade as observed in plain radiograph, MRI, histology, and immunohistochemistry. We suggest that needle size affects the occurrence of progression of degeneration; thus, the large needle size was required to accelerate the deterioration. The size of needle and time point after injury should be considered when investigating the effect of therapeutic materials to retard degeneration or regenerate the intervertebral disc. Published by Elsevier Inc.
引用
收藏
页码:1014 / 1023
页数:10
相关论文
共 42 条
  • [1] Biological repair of intervertebral disc
    An, HS
    Thonar, EJMA
    Masuda, K
    [J]. SPINE, 2003, 28 (15) : S86 - S92
  • [2] The relationship between apoptosis of endplate chondrocytes and aging and degeneration of the intervertebral disc
    Ariga, K
    Miyamoto, S
    Nakase, T
    Okuda, S
    Meng, WX
    Yonenobu, K
    Yoshikawa, H
    [J]. SPINE, 2001, 26 (22) : 2414 - 2420
  • [3] Bland JM, 1996, BRIT MED J, V313, P744
  • [4] Statistics notes: Measurement error proportional to the mean .23.
    Bland, JM
    Altman, DG
    [J]. BRITISH MEDICAL JOURNAL, 1996, 313 (7049) : 106 - 106
  • [5] A STUDY OF THE EFFECTS OF BIPEDISM AND UPRIGHT POSTURE ON THE LUMBOSACRAL SPINE AND PARAVERTEBRAL MUSCLES OF THE WISTAR RAT
    CASSIDY, JD
    YONGHING, K
    KIRKALDYWILLIS, WH
    WILKINSON, AA
    [J]. SPINE, 1988, 13 (03) : 301 - 308
  • [6] A COEFFICIENT OF AGREEMENT FOR NOMINAL SCALES
    COHEN, J
    [J]. EDUCATIONAL AND PSYCHOLOGICAL MEASUREMENT, 1960, 20 (01) : 37 - 46
  • [7] The effect of relative needle diameter in puncture and sham injection animal models of degeneration
    Elliott, Dawn M.
    Yerramalli, Chandra S.
    Beckstein, Jesse C.
    Boxberger, John I.
    Johannessen, Wade
    Vresilovic, Edward J.
    [J]. SPINE, 2008, 33 (06) : 588 - 596
  • [8] Biomechanics of the aging spine
    Ferguson, SJ
    Steffen, T
    [J]. EUROPEAN SPINE JOURNAL, 2003, 12 (Suppl 2) : S97 - S103
  • [9] A CADAVERIC STUDY COMPARING DISCOGRAPHY, MAGNETIC-RESONANCE-IMAGING, HISTOLOGY, AND MECHANICAL-BEHAVIOR OF THE HUMAN LUMBAR-DISK
    GUNZBURG, R
    PARKINSON, R
    MOORE, R
    CANTRAINE, F
    HUTTON, W
    VERNONROBERTS, B
    FRASER, R
    [J]. SPINE, 1992, 17 (04) : 417 - 426
  • [10] A simple disc degeneration model induced by percutaneous needle puncture in the rat tail
    Han, Bin
    Zhu, Kai
    Li, Fang-cai
    Xiao, Yu-xiang
    Feng, Jie
    Shi, Zhong-li
    Lin, Min
    Wang, Jun
    Chen, Qi-xin
    [J]. SPINE, 2008, 33 (18) : 1925 - 1934