Properties of Procoagulant Platelets Defining and Characterizing the Subpopulation Binding a Functional Prothrombinase

被引:57
作者
Fager, Ammon M. [1 ]
Wood, Jeremy P. [1 ]
Bouchard, Beth A. [1 ]
Feng, Ping [1 ]
Tracy, Paula B. [1 ]
机构
[1] Univ Vermont, Dept Biochem, Coll Med, Burlington, VT 05405 USA
关键词
adhesion molecules; coagulation; platelets; thrombin; factor V; COAGULATION-FACTOR-V; ACTIVATED PLATELETS; ALPHA-THROMBIN; OLD PLATELETS; FACTOR-X; IN-VIVO; PROTEINS; SURFACE; HETEROGENEITY; RECEPTORS;
D O I
10.1161/ATVBAHA.110.216531
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective-The goal of this study was to define and characterize the subpopulation of platelets capable of regulating the functional interactions of factors Va (FVa) and Xa (FXa) on the thrombin-activated platelet surface. Methods and Results-Flow cytometric analyses were used to define and characterize platelet subpopulations. At a concentration of thrombin known to elicit maximal platelet activation, platelet-derived FVa release, and prothrombinase assembly/function, only a subpopulation of platelets was positive for FVa and FXa binding. An additional subpopulation bound lower levels of FVa but little, if any, FXa. Fluorescence microscopy analyses confirmed these data. Phenotypically, platelets capable of binding FXa were more highly reticulated and demonstrated significantly increased expression of several key adhesion molecules, including P-selectin, glycoprotein Ib alpha, and integrins alpha(IIb) and beta(3). This platelet subpopulation was also defined by the expression of a nondissociable, membrane-bound pool of functional platelet-derived FVa, which made up approximate to 35% to 50% of the total membrane-bound cofactor. Conclusion-The ability of activated platelets to support thrombin generation is defined by a subpopulation of platelets expressing a nondissociable pool of platelet-derived FVa and increased adhesive receptor density. This subpopulation is hypothesized to play a significant role in regulating both normal hemostasis and pathological thrombus formation because the adherent properties of platelets and their ability to mount and sustain a procoagulant response are crucial steps in both of these processes. (Arterioscler Thromb Vasc Biol. 2010;30:2400-2407.)
引用
收藏
页码:2400 / U142
页数:17
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