Trehalose accumulation from soluble starch by Saccharomycopsis fibuligera sdu

被引：101

作者：

Chi, ZM ^{[1
]}

Liu, J ^{[1
]}

Zhang, W ^{[1
]}

机构：

[1] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Shandong, Peoples R China

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2001年 / 28卷 / 2-3期

关键词：

trehalose accumulation; soluble starch; Saccharomycopsis fibuligera; agitation speed;

D O I：

10.1016/S0141-0229(00)00318-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Trehalose accumulation from starch by Saccharomycopsis fibuligera sdu was examined in 300-ml shaken flask culture and Biostat B-2 2-1 fermentation. In the 300-ml flask. 16.5% (w/w) trehalose accumulated in the yeast cells (cell dry weight) was observed with 100-ml medium shaken at 200 rpm for 50 h at 30 degreesC. We found that 1.0% soluble starch in the medium was most suitable for trehalose accumulation by this yeast strain. In the Biostat B-2 2-1 fermenter, 18.0% (w/w) trehalose accumulated in the yeast cells (cell dry weight) was observed within 48 h of fermentation when agitation speed was 200 rpm. The trehalose obtained from the yeast cells was identical to standard trehalose from Sigma based on the analysis results of High-Performance Exchange Anionic Chromatography (HPEAC).

引用

页码：240 / 245

页数：6

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