Ultra-high resolution imaging of DNA and nucleosomes using non-contact atomic force microscopy

被引：31

作者：

Davies, E

Teng, KS

Conlan, RS

Wilks, SP

机构：

[1] Univ Coll Swansea, Sch Engn, Ctr Multidisciplinary Nanotechnol, Swansea SA2 8PP, W Glam, Wales

[2] Univ Coll Swansea, Sch Biol Sci, Mol Biol Res Grp, Swansea SA2 8PP, W Glam, Wales

来源：

FEBS LETTERS | 2005年 / 579卷 / 07期

关键词：

MeSH : DNA; nucleosome; microscopy; atomic force; molecular structure; molecular biology; nanotechnology;

D O I：

10.1016/j.febslet.2005.02.028

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Visualisation of nano-scale biomolecules aids understanding and development in molecular biology and nanotechnology. Detailed structure of nucleosomes adsorbed to mica has been captured in the absence of chemical-anchoring techniques, demonstrating the usefulness of non-contact atomic force microscopy (NC-AFM) for ultra-high resolution biomolecular imaging. NC-AFM offers significant advantages in terms of resolution, speed and ease of sample preparation when compared to techniques such as cryo-electron microscopy and X-ray crystallography. In the absence of chemical modification, detailed structure of DNA deposited on a gold substrate was observed for the first time using NC-AFM, opening up possibilities for investigating the electrical properties of unmodified DNA. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1702 / 1706

页数：5