Long Non-Coding RNA DUXAP8 Enhances Renal Cell Carcinoma Progression via Downregulating miR-126

被引:49
作者
Huang, Tao [1 ]
Wang, Xiao [1 ]
Yang, Xiaokun [2 ]
Ji, Jianlei [1 ]
Wang, Qinghai [1 ]
Yue, Xuan [1 ]
Dong, Zheng [1 ]
机构
[1] Qingdao Univ, Affiliated Hosp, Dept Kidney Transplantat, Qingdao, Shandong, Peoples R China
[2] Qingdao Univ, Affiliated Hosp, Dept Urol, Qingdao, Shandong, Peoples R China
来源
MEDICAL SCIENCE MONITOR | 2018年 / 24卷
关键词
Carcinoma; Renal Cell; MicroRNAs; RNA; Long Noncoding; CANCER; PROLIFERATION; MICRORNAS; INVASION; MIGRATION; TUMORS;
D O I
10.12659/MSM.910054
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Background: Renal cell carcinoma (RCC) is one of the common malignant tumors in the urinary system, which endangers human health for a long time. The past decade, the molecular biology of renal cell carcinoma has made considerable progress, so that we have a more profound understanding of renal cell carcinoma. Molecular biological mechanism of renal cell carcinoma remains to be explored. Evidence indicates that long non-coding RNAs (lncRNAs) may be important players in human cancer progression, including RCC. In this study, we found that a newly discovered pseudogene-derived lncRNA named DUXAP8, a 2107-bp RNA, was remarkably upregulated in RCC. Material/Methods: Expression of lncRNA DUXAP8 was determined by a qRT-PCR assay in RCC tissues. The proliferation and invasion of RCC cell were measured by a cell proliferation assay and a Transwell invasion assay. Expression of miR-126 was detected by real-time PCR. Interactions between lncRNA DUXAP8 and miR-126 were measured by a luciferase reporter assay and an RNA-pull down assay. In vivo experiments were used to detect tumor formation. Results: Together, our study not only identifies lncRNA DUXAP8 as a negative regulator of renal cancer with potential clinical value, but also reveals a regulatory mechanism by long non-coding RNAs to control tumor development. Conclusions: Results from this study provide evidence that lncRNA DUXAP8 enhances renal cell carcinoma progression via downregulating of miR-126, which offers a new approach for the treatment of RCC.
引用
收藏
页码:7340 / 7347
页数:8
相关论文
共 50 条
  • [21] Long Non-Coding RNA CRNDE Promotes Colorectal Carcinoma Cell Progression and Paclitaxel Resistance by Regulating miR-126-5p/ATAD2 Axis
    Liu, Chang
    Hou, Jianfeng
    Shan, Fengxiao
    Wang, Lijuan
    Lu, Hanjie
    Ren, Tiejun
    ONCOTARGETS AND THERAPY, 2020, 13 : 4931 - 4942
  • [22] Long non-coding RNA PTCSC3 suppresses triple-negative breast cancer by downregulating long non-coding RNA MIR100HG
    Zhang, Guojun
    Gao, Lei
    Zhang, Junliang
    Wang, Rui
    Wei, Xiangdong
    ONCOLOGY LETTERS, 2023, 26 (02)
  • [23] The Long Noncoding RNA DUXAP8 Facilitates the Malignant Progression of Colon Cancer via the microRNA-378a-3p/FOXQ1 Axis
    Shang, Rui
    Jin, Jianqin
    Wang, Yuecheng
    GUT AND LIVER, 2025, 19 (02) : 219 - 235
  • [24] The long non-coding RNA NEAT1 enhances epithelial-to-mesenchymal transition and chemoresistance via the miR-34a/c-Met axis in renal cell carcinoma
    Liu, Fei
    Chen, Na
    Gong, Yanchun
    Xiao, Ruihai
    Wang, Weichao
    Pan, Zhengyue
    ONCOTARGET, 2017, 8 (38) : 62927 - 62938
  • [25] Long non-coding RNA DUXAP9 promotes hepatocellular carcinoma cell stemness via directly interacting with sox9
    Zeng, Ru
    Wang, Chunyue
    Wang, Wenyi
    Wang, Shuangjia
    ENVIRONMENTAL TOXICOLOGY, 2021, 36 (09) : 1793 - 1801
  • [26] Long non-coding RNA HCG18 facilitates the progression of laryngeal and hypopharyngeal squamous cell carcinoma by upregulating FGFR1 via miR-133b
    Peng, Hongbin
    Ge, Pingjiang
    MOLECULAR MEDICINE REPORTS, 2022, 25 (02)
  • [27] Upregulation of long non-coding RNA MALAT1 correlates with tumor progression and poor prognosis in clear cell renal cell carcinoma
    Zhang, Hai-min
    Yang, Feng-qiang
    Chen, Shao-Jun
    Che, Jianping
    Zheng, Jun-hua
    TUMOR BIOLOGY, 2015, 36 (04) : 2947 - 2955
  • [28] Long non-coding RNA CASC9 promotes the progression of retinoblastoma via interacting with miR-145-5p
    Zhang, Ting
    Yang, Jingpu
    Gong, Fangchao
    Li, Lin
    Li, Aipeng
    CELL CYCLE, 2020, 19 (18) : 2270 - 2280
  • [29] Role of Metabolic Reprogramming of Long non-coding RNA in Clear Cell Renal Cell Carcinoma
    Zhang, Huijie
    Yu, Lei
    Chen, Jing
    Liu, Liting
    Yang, Xudong
    Cui, Hongwei
    Yue, Genquan
    JOURNAL OF CANCER, 2022, 13 (02): : 691 - 705
  • [30] Long non-coding RNA HOTTIP promotes renal cell carcinoma progression through the regulation of the miR-615/IGF-2 pathway
    Wang, Qifei
    Wu, Guangzhen
    Zhang, Zhiwei
    Tang, Qizhen
    Zheng, Wei
    Chen, Xiaochi
    Chen, Feng
    Li, Quanlin
    Che, Xiangyu
    INTERNATIONAL JOURNAL OF ONCOLOGY, 2018, 53 (05) : 2278 - 2288