Synthesis of poly(3-hydroxybutyrate) by immobilized poly(3-hydroxybutyrate) synthase

被引：5

作者：

Tajima, K ^{[1
]}

Tannai, H ^{[1
]}

Satoh, Y ^{[1
]}

Munekata, M ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Engn, Div Mol Chem, Sapporo, Hokkaido 0608628, Japan

来源：

POLYMER JOURNAL | 2003年 / 35卷 / 04期

关键词：

poly(3-hydroxybutyrate); PHB; immobilized enzyme; PHB synthase;

D O I：

10.1295/polymj.35.407

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The synthesis of poly(3-hydroxybutyrate) (PHB) by immobilized poly(3-hydroxybutyrate) synthase (PhbC) was discussed. The enzymes used for the monomer synthesis and PhbC were purified and immobilized on the carrier resin Chitopal BCW2603. For immobilization, the purified enzymes were mixed with 500 mg of wet resin in 100 mM Tris-HCl at 4 °C for 2 h. The results obtained suggested that PHB can be polymerized on the surface of carrier resin by immobilized PHB synthase.

引用

页码：407 / 410

页数：4

共 50 条

[41] Effect of Cultivation Conditions on Poly(3-hydroxybutyrate) Synthesis by Nodule Bacteria Rhizobium phaseoli [J].

Kosmachevskaya, O. V. ;

Osipov, E. V. ;

Van Chi, Tran ;

Mai, Pham Thi Tuyet ;

Topunov, A. F. .

APPLIED BIOCHEMISTRY AND MICROBIOLOGY, 2020, 56 (01) :64-71

[42] Plant oil fillers toughened poly(3-hydroxybutyrate) green biocomposites [J].

Muiruri, Joseph Kinyanjui ;

Yeo, Jayven Chee Chuan ;

Run, Hong ;

Lin, Ting Ting ;

Hou, Xunan ;

Raveenkumar, Vijayakumar ;

Jian, Boo Yi ;

Thitsartarn, Warintorn ;

He, Chaobin ;

Li, Zibiao .

EUROPEAN POLYMER JOURNAL, 2024, 210

[43] Annatto-colored Poly(3-hydroxybutyrate): A Comprehensive Study on Photodegradation [J].

Caroline Salvan Pagnan ;

Artur Caron Mottin ;

Rodrigo Lambert Oréfice ;

Eliane Ayres ;

Jairo José Drummond Câmara .

Journal of Polymers and the Environment, 2018, 26 :1169-1178

[44] A poly(3-hydroxybutyrate)-chitosan polymer conjugate for the synthesis of safer gold nanoparticles and their applications [J].

Silvestri, Daniele ;

Waclawek, Stanislaw ;

Sobel, Bartlomiej ;

Torres-Mendieta, Rafael ;

Novotny, Vit ;

Nguyen, Nhung H. A. ;

Sevcu, Alena ;

Padil, Vinod V. T. ;

Muellerova, Jana ;

Stuchlik, Martin ;

Papini, Marco Petrangeli ;

Cernik, Miroslav ;

Varma, Rajender S. .

GREEN CHEMISTRY, 2018, 20 (21) :4975-4982

[45] Influence of redox potential on the accumulation of poly(3-hydroxybutyrate) by Bacillus megaterium [J].

Hassemer, Guilherme de Souza ;

do Nascimento, Lucas Henrique ;

Lin, Yen-Han ;

Steffens, Clarice ;

Junges, Alexander ;

Valduga, Eunice .

BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2023, 46 (08) :1221-1230

[46] Evaluation of poly(3-hydroxybutyrate) production by microbiota endogenous to oleaginous plants [J].

Das, Rituparna ;

Pal, Arundhati ;

Paul, Amal Kanti .

MALAYSIAN JOURNAL OF MICROBIOLOGY, 2019, 15 (01) :76-87

[47] Biobased Poly(3-hydroxybutyrate)/Starch/Cellulose Nanofibrils for Nutrients Coatings [J].

Franca, Debora ;

Angelo, Luciana M. ;

Souza, Claudinei F. ;

Faez, Roselena .

ACS APPLIED POLYMER MATERIALS, 2021, 3 (06) :3227-3237

[48] EXTRACTION OF POLY(3-HYDROXYBUTYRATE), PRODUCED BY Cupriavidus necator, WITH PROPYLENE CARBONATE [J].

Quines, Luci K. M. ;

Ienczak, Jaciane L. ;

Schmidt, Melodi ;

Zanfonato, Kellen ;

Rodrigues, Maria I. ;

Schmidell, Willibaldo ;

Aragao, Glaucia M. F. .

QUIMICA NOVA, 2015, 38 (02) :214-220

[49] Estimation of the Mechanical Properties of Poly(3-hydroxybutyrate) from IR Data [J].

Polyak, Peter ;

Mackei, Fruzsina ;

Toth, Agoston ;

Bredacs, Marton .

JOURNAL OF POLYMERS AND THE ENVIRONMENT, 2023, 31 (12) :5185-5197

[50] Microscopical investigation of poly(3-hydroxybutyrate) granule formation in Azotobacter vinelandii [J].

Hermawan, Siska ;

Jendrossek, Dieter .

FEMS MICROBIOLOGY LETTERS, 2007, 266 (01) :60-64

← 1 2 3 4 5 →