Synthesis of poly(3-hydroxybutyrate) by immobilized poly(3-hydroxybutyrate) synthase

被引：5

作者：

Tajima, K ^{[1
]}

Tannai, H ^{[1
]}

Satoh, Y ^{[1
]}

Munekata, M ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Engn, Div Mol Chem, Sapporo, Hokkaido 0608628, Japan

来源：

POLYMER JOURNAL | 2003年 / 35卷 / 04期

关键词：

poly(3-hydroxybutyrate); PHB; immobilized enzyme; PHB synthase;

D O I：

10.1295/polymj.35.407

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The synthesis of poly(3-hydroxybutyrate) (PHB) by immobilized poly(3-hydroxybutyrate) synthase (PhbC) was discussed. The enzymes used for the monomer synthesis and PhbC were purified and immobilized on the carrier resin Chitopal BCW2603. For immobilization, the purified enzymes were mixed with 500 mg of wet resin in 100 mM Tris-HCl at 4 °C for 2 h. The results obtained suggested that PHB can be polymerized on the surface of carrier resin by immobilized PHB synthase.

引用

页码：407 / 410

页数：4

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