Cyclophilin a modulates processing of human immunodeficiency virus type 1 p55Gag:: Mechanism for antiviral effects of cyclosporin A

被引：47

作者：

Streblow, DN

Kitabwalla, M

Malkovsky, M

Pauza, CD

机构：

[1] Univ Wisconsin, Dept Pathol & Lab Med, Madison, WI 53706 USA

[2] Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA

来源：

VIROLOGY | 1998年 / 245卷 / 02期

关键词：

D O I：

10.1006/viro.1998.9155

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The molecular chaperone cyclophilin A (Cyp A) modulates human immunodeficiency virus type 1 (HIV-1) infectivity through its interactions with Gag structural proteins. The molecular mechanism for CypA in HIV-1 replication is not known. We studied chaperone effects on Gag precursor processing using cyclosporin A (CsA) to bind CypA and prevent its interaction with p55(Gag). CsA treatment inhibited p55(Gag) processing in extracellular virus-like particles produced from COS cells. We confirmed the effect of CsA on Gag processing by examining virions produced from CEMx174 cells infected with HIV-1(LAI). Particles accumulated in the presence of CsA displayed mostly immature virion morphology and lacked condensed capsids. CsA has a direct effect on HIV-1 Gag processing that implicates CypA as having an important role in the maturation of HIV-I particles. (C) 1998 Academic Press.

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页码：197 / 202

页数：6

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